持续正加速度对实验性大鼠胃溃疡愈合质量的影响  

Effect of persistent positive acceleration on the quality of ulcer healing in rats with experimental gastric ulcer

作　　者：陈璐[1] 陈英[1] 范勤[1] 韩全利[1] 唐合兰[1] 李静[1] 杨春敏[1] 

机构地区：[1]安徽医科大学中国人民解放军空军总医院临床学院东楼消化科,北京市100142

出　　处：《世界华人消化杂志》2013年第19期1841-1846,共6页World Chinese Journal of Digestology

基　　金：全军十二五后勤科研计划基金资助项目;No.AKJ11J004~~

摘　　要：目的:探讨正加速度(+Gz)对消化性溃疡模型大鼠的溃疡愈合的影响及其机制.方法:将32只♂SD大鼠随机分为对照组、+5Gz组、+10Gz组、和+10Gz+康复新液组,每组8只.采用乙酸烧灼法建立大鼠胃溃疡模型,造模后3d加速度隔日处理1wk,每次持续5min,共4次.+10Gz+康复新液组同时予以康复新液2mL灌胃1wk,1wk后取其组织及血液标本.运用放射免疫法检测胃黏膜前列腺素E2(prostaglanding E2,PGE2)、血清降钙素基因相关肽(calcitonin gene-related peptide,CGRP),运用硝酸还原酶法检测血清一氧化氮(nitric oxide,NO).结果:随+Gz值增高,光镜下再生黏膜厚度变薄,扩张腺体数目增多.大鼠胃黏膜PGE2含量对照组为6.986pg/mL±0.743pg/mL,+5Gz组为5.147pg/mL±0.652pg/mL,+10Gz组为3.438pg/mL±0.908pg/mL,+10Gz+康复新液组为6.133pg/mL±0.545pg/mL.+10Gz组较+5Gz组和对照组低(P<0.01),并且低于+10Gz+康复新液组(P<0.01).大鼠血清(CGRP)含量对照组为82.583pg/mL±11.788pg/mL,+5Gz组为78.333pg/mL±11.290pg/mL,+10Gz组为62.254pg/mL±15.943pg/mL,+10Gz+康复新液组为78.455pg/mL±12.645pg/mL.+10Gz组较+5Gz组和对照组低(P<0.05),并且较+10Gz+康复新液组低(t=-2.252,P<0.05).大鼠血清NO含量对照组为53.806mol/L±9.272mol/L,+5Gz组为47.783mol/L±2.847mol/L,+10Gz组为44.773mol/L±6.858mol/L,+10Gz+康复新液组为53.853mol/L±7.372mol/L.+10Gz组较+5Gz组和对照组低(P<0.05),并且较+10Gz+康复新液组低(t=-2.551,P<0.05).结论:+Gz条件下,溃疡愈合延迟;康复新液灌胃可减轻胃黏膜的损伤,促进溃疡愈合.AIM： To investigate the effect of positive accel- eration （＋Gz） on gastric ulcer healing in rats and to explore the possible mechanisms involved. METHODS： Thirty-two male SD rats were ran- domly and equally divided into a control group, a ＋5Gz exposure group, a ＋10Gz exposure group, and a ＋10Gz exposure plus KangFuXin solution group. Gastric ulcer was induced in rats with ace- tic acid. After 3 d, rats in the latter three groups were exposed to ＋Gz each other day for 1 wk（four times in all, five minutes each time）. The rats in the ＋10Gz exposure plus KangFuXin solu- tion group were additionally gavaged with 2 mL of KangFuXin solution every day for 1 wk. Tis- sue and bloods samples were taken to detect the contents of prostaglandin E2 （PGE2） in the gas- tric mucosa and calcitonin gene-related peptide （CGRP） in blood by radioimmunoassay and the content of nitric oxide （NO） in blood by nitrate reductase assay. RESULTS： The higher ＋Gz, the thinner restored mucosa thickness and the more the number of cystically dilated glands. The contents of PGE2 in the gastric mucosa were significantly lower in the ＋10Gz exposure group than in the ＋5Gz exposure group, control group, and ＋10Gz ex- posure plus KangFuXin solution group （3.438 pg/mL ±0.908 pg/mL vs 5.147 pg/mL ±0.652 pg/mL, 6.986 pg/mL ±0.743 pg/mL, 6.133 pg/ mL ±0.545 pg/mL, all P 〈 0.01）. The contents of CGRP in blood were significantly lower in the ＋10Gz exposure group than in the ＋5Gz expo- sure group, control group, and ＋10Gz exposure plus KangFuXin solution group （62.254 pg/mL ±15.943 pg/mL vs 78.333 pg/mL ±11.290 pg/ mL, 82.583 pg/mL ±11.788 pg/mL, 78.455 pg/ mL±12.645 pg/mL, all P 〈 0.05）. The contents of NO in blood were significantly lower in the ＋10Gz exposure group than in the ＋5Gz expo- sure group, control group, and ＋10Gz exposure plus KangFuXin solution group （44.773 μmol/L ±6.858 μmol/L vs 47.783 μmol/L ±2.847 μmol/L, 53.806 μmol/L ±9.272 μmol/L, 53.853 μmol/L ±7.3

关 键 词：加速度 消化性溃疡 前列腺素E2 降钙素基因相关肽 一氧化氮 溃疡愈合质量 

分 类 号：R573.1[医药卫生—消化系统]