固定Ionic liquid/Silica sol/Nafion膜中的联吡啶钌电化学发光行为的研究及应用  

Electrochemiluminescence Based on Ionic Liquid/Silica Sol/Nafion-Tris(2,2′-bipyridine)ruthenium(Ⅱ) Film Modified Gold Electrode and Its Analytical Application

作　　者：李利军[1] 杨兰兰[1] 罗应[1] 张明涛[2] 张瑞瑞[1] 崔福海[1] 孙科[1] 李彦青[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]河北科技大学建筑工程学院,河北石家庄050000

出　　处：《分析测试学报》2013年第7期817-822,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(31060219);广西科学基金资助项目(0640029)

摘　　要：制备了Ionic liquid/Silica sol/Nafion-联吡啶钌复合物膜修饰金电极。采用循环伏安(CV)和电致化学发光(ECL)法,考察了联吡啶钌和左氧氟沙星在此修饰电极上的电化学及其发光行为。结果表明,此修饰电极表现出很好的电化学活性和电化学发光响应。基于左氧氟沙星对固定化联吡啶钌在0.1 mol/L磷酸盐缓冲液中的弱电化学发光信号有较强的增敏作用,建立了电化学发光检测盐酸左氧氟沙星的新方法。在最佳实验条件下,左氧氟沙星浓度在1.0×10-7～1.0×10-4mol/L范围内与相对发光强度呈线性关系(r2=0.998 4),检出限(S/N=3)为1.59×10-9mol/L。连续平行测定2.2×10-5mol/L的左氧氟沙星溶液10次,发光强度的相对标准偏差(RSD)为3.6%。对样品进行回收率试验,回收率在95.6%～103.5%之间,RSD(n=5)为3.0%。该方法具有较高的选择性和灵敏度,样品处理简单快速,用于盐酸左氧氟沙星的测定,结果满意。An ionic liquid/Silica sol/Nafion-Ru（bpy）2＋3 modified gold electrode was prepared.The electrochemical and ECL characteristies of Ru（bpy）2＋3 and levofloxacin at the modified electrode were studied,and the modified electrode exhibits good electrochemical activity and ECL response.Based on the obvious electrochemiluminescence sensitization effect of levofloxacin with Ru（bpy）2＋3 in 0.1mol/L phosphate buffer,a new electrochemiluminescence method for the determination of levofloxacin hydrochloride was developed.Under the optimum condition,the calibration curve showed a good linearity with levofloxacin concentration in the range of 1.0×10-7-1.0×10-4 mol/L（r2=0.998 4） and the detection limit（S/N=3） was 1.59 ×10-9 mol/L.The RSD for 10 times determination of 2.2×10-5 mol/L levofloxacin was 3.6 %.The recoveries were in the range of 95.6%-103.5% and the RSD（n=5） was 3.0 %.The proposed method showed high selectivity,sensitivity and simplicity.This method was applied in the analysis of levofloxacin hydrochloride tablet with satisfactory results.

关 键 词：电化学发光 联吡啶钌 硅溶胶 离子液体 左氧氟沙星 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]