多杀菌素高产菌株快速筛选方法的研究  

Rapid screening method of high yield strains spinosad

作　　者：陈园[1,2] 熊犍[1] 王超[2] Eddie Chio 邹球龙[2] 张晓琳[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510641 [2]国家粮食局科学研究院,北京100037

出　　处：《粮油食品科技》2013年第4期99-102,共4页Science and Technology of Cereals,Oils and Foods

基　　金："十二五"国家科技支撑计划课题(2011BAD03B02-2)

摘　　要：研究利用多杀菌素对蚊子幼虫致死率与其浓度的相关性,以蚊子幼虫为靶标动物建立基于活体筛选的多杀菌素高产菌株高通量快速筛选模型。通过比较蚊子幼虫的致死率筛选获得多杀菌素产量较高的菌株。结果显示:2龄埃及伊蚊幼虫具有高的敏感性和精确性,生测曲线拟合获得的毒力回归方程标准误差(SE)较小,最适宜作为供试虫源用于快速初筛模型。方法能显著提高多杀菌素高产菌株的筛选效率。A fast and high flux screening model for spinosad high yield strain was established,which made use of the relevance between the fatality rate of mosquito larvae and the concentration of spinosad. The spinosad high yield strain was obtained by comparing the fatality rate of mosquito larvae. The results showed that the 2nd instar of mosquitoes were the most suitable for the insect source used for rapid screen- ing model, as it has high sensitivity, accuracy and little standard error of virulence regression equation which was acquired by curve fitting in bioassay test. The method can significantly improve the screening efficiency of spinosad high yield strain.

关 键 词：刺糖多孢菌 多杀菌素 通量 生物检测 

分 类 号：TS201.3[轻工技术与工程—食品科学]