萝卜硫素增加HL-60和人原代白血病细胞对化疗药敏感性实验研究  

Sulforaphane Increased Chemotherapy Sensitivity of HL-60 Cell Line and Human Primary Leukemia Cells

作　　者：许晓峰[1] 张学进[1] 程汝滨[2] 高瑞兰[3] 

机构地区：[1]浙江省中西医结合医院肿瘤血液科,杭州310003 [2]浙江中医药大学药学院 [3]浙江省中医院血液病研究所

出　　处：《浙江中西医结合杂志》2013年第7期526-529,534,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2010ZB113)

摘　　要：目的:探讨萝卜硫素(SF)对白血病细胞化疗药物敏感性的作用。方法:琼脂半固体集落培养法和MTT法检测不同浓度的萝卜硫素对HL-60细胞增殖的影响及协同化疗药对细胞生长的抑制作用;PHA-LCM液相-半固体二步培养SF协同化疗药抑制人原代白血病细胞生长。结果:琼脂半固体集落培养法和MTT法证实在一定浓度范围内SF对HL-60细胞增殖抑制(P<0.05,P<0.01),呈剂量依赖性,SF能够增强HL-60细胞对化疗药物高三尖杉酯碱(Hom)和阿糖胞苷(Ara-c)敏感性(P<0.05);PHA-LCM液相-半固体二步培养法证实SF在一定浓度范围内对人原代白血病细胞有抑制作用(P<0.05,P<0.01),呈剂量依赖性,并增加人原代白血病细胞对化疗药Hom和Ara-c敏感性。结论:萝卜硫素在一定浓度下有效抑制白血病细胞增殖,并能增强化疗药物的敏感性。Objective： To investigate the effect of sulforaphane（SF） on chemotherapy sensitivity of leukemia cells.Methods： Semi-solid agar colony culture assay and MMT method were used to observe the effect of different concentrations of SF on HL-60 cell proliferation and homoharringtonine＋ ara-c chemotherapy on cell growth.PHALCM two-stage culture method was used to detect the effect of SF and homoharringtonine＋ara-c chemotherapy on human primary leukemia cells.Results： With a certain range of concentrations,SF inhibited the proliferation of HL-60 cells and the growth of human primary leukemia cells in a dose-dependent manner（P〈0.05,P〈0.01）.SF enhanced homoharringtonine＋ara-c chemotherapy sensitivity of HL-60 cells（P〈0.05） and human primary leukemia cells.Conclusion： SF can inhibit the proliferation of leukemia cells with a certain range of concentrations and enhance chemotherapy sensitivity of these cells.

关 键 词：人原代白血病细胞 萝卜硫素 化疗药 敏感性 

分 类 号：R733.7[医药卫生—肿瘤]