CD44及CD44v6在不同分化程度食管癌中的表达水平  

The expression of CD44 and CD44v6 on esophageal carcinoma in differential degree

作　　者：郑淑妍[1] 陈强[1] 黄小萍[1] 苏少雪[1] 林敏[1] 刘俊斌[1] 杨立业[1] 

机构地区：[1]南方医科大学附属潮州市中心医院,广东潮州521000

出　　处：《分子诊断与治疗杂志》2013年第4期232-235,共4页Journal of Molecular Diagnostics and Therapy

基　　金：广东省医学科学技术研究基金(B2008179);广东省社会发展计划(2011B031800329)

摘　　要：目的通过免疫组化研究CD44及其变异体CD44v6在食管癌中的表达情况,探讨其与食管癌的关系及临床意义。方法收集潮州市中心医院病理科存档的食管癌蜡块(所有病例术前均未接受过放、化疗)。采用免疫组化PV-9000二步法(非生物素)检测食管癌中CD44的表达共145例,CD44v6共146例。SPSS16.0统计软件分析实验结果。对于各组之间表达情况的数据统计采用多独立样本非参数检验:Kruskal-Wallis H检验;各组中两两比较采用两独立样本非参数检验:Kruskal-Wallis H检验的方法进行比较。结果 CD44及CD44v6在5例小细胞癌中均不表达0%,在鳞状细胞癌中的阳性表达率分别为90.71%(127/140)和82.27%(116/141)。其中高分化鳞状细胞癌组分别为100%(10/10)及88.89%(8/9),中分化组分别为98.21%(55/56)及94.74%(54/57),而低分化组则分别为83.78%(62/74)及72%(54/75)。CD44及CD44v6在鳞状细胞癌与小细胞癌之间的表达情况均有显著差异(均P<0.01),在高分化组与中分化组间的表达差别无统计学意义(P>0.05),高分化组与低分化组及中分化组与低分化组间差别均有统计学意义(均P<0.05),分化越好,CD44及CD44v6的表达越强。结论 CD44及CD44v6在食管癌中的表达与肿瘤的组织学分型及鳞状细胞癌的分化程度有关,提示它们可以作为食管癌组织学分型的一个辅助标记物及预后预测因子之一。另外,鉴于CD44及CD44v6在食管癌中的高表达率,提示其可能为食管癌干细胞的标志物。Objective To explore the relationship and clinical significance through investigated the expression of CD44 and CD44v6 in esophageal carcinoma by immunohistochemical method. Mothods Paraffin blocks of esophageal carcinoma were collected from department of Pathology, Chaozhou central hospital. The patients were not treated with chemotherapy or radiotherapy before operation. The expression of CD44 in 145 cases were detected by immunohistochemistry PV-9000 two-steps methods with monoclonal antibodies directed against specific CD44. The expression of CD44v6 in 146 cases were detected by the same method. The results were analyzed with SPSS 16.0 statistics software. The difference of expression between each sample in one group was analyzed by multi-independent samples nonparameter tests （Kruskal-Wallis H tests）. The difference of expression between two samples in one group was analyzed by 2-independent samples nonparameter tests （Kruskal-Wallis H tests）. Results The expression of CD44 and CD44v6 in five cases of small cell carcinoma were all negative. In squamous cell carcinoma （SCC）, the positive expression rate were 90.71% （127/140） and 82.27% （116/141） each other. Among them, the positive rate of the well-differentiated SCC were 100% （10/10） and 88.89% （8/9）, respectively. The moderately differentiated SCC were 98.21% （55/56） and 94.74% （54/57）, respectively. The poorly differentiated SCC were 83.78% （62/74） and 72% （54/75）, respectively. There was significant difference between SCC and small cell carcinoma for the expression of CD44 and CD44v6 （P〈0.01）. There was no significant difference for the expression of CD44 and CD44v6 between well-differentiated SCC and moderately differentiated SCC （P〉0.05）. But it was found that there was significant difference between well-differentiated and poorly differentiated SCC （P〈0.01）. The same result was found between moderately differentiated SCC and poorly differentiated SCC too （P〈0.01）. The better differenti

关 键 词：CD44 CD44V6 免疫组化 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]