采取高效液相色谱法测定植物样品中花青素的研究  

Study of Anthocyanins Determined in Plant Samples by High Performance Liquid Chromatography

作　　者：刘红[1] 徐科[1] 

机构地区：[1]贵阳医学院化学教研室,贵州贵阳550004

出　　处：《安徽农业科学》2013年第14期6123-6124,共2页Journal of Anhui Agricultural Sciences

基　　金：黔科合SY字[2011]3128

摘　　要：[目的]建立应用高效液相色谱、紫外检测器同时测定植物样品中花青素的测定方法。[方法]样品采用乙醇酸性水溶液(乙醇∶水∶盐酸=3∶1∶1)振荡,超声提取,沸水浴水解,用高效液相色谱紫外检测器测定。流动相A为浓度1%甲酸水溶液,流动相B为浓度1%甲酸乙腈溶液,流速0.8 ml/min,检测波长530 nm。[结果]样品添加回收率为75.7%～92.7%,方法精密度为2.7%～6.3%,当浓度为1～200μg/ml时峰面积与浓度呈线性关系,最低检出限为0.03～0.11 mg/kg,最低定量限为0.10～0.35 mg/kg。[结论]该方法简便快速,稳定性好,可作为植物样品中花青素测定的有效方法。[ Objective] The research aimed to establish the simultaneous determination method for the anthocyanins in plant samples by high performance liquid chromatography/UV detector. [ Method] Sample was extracted with ethanol in acidic aqueous solution（ V（C2HsOH） ： V （ H2 O） ： V（HC1） = 3：1 ： 1 ） and ultrasonic extraction, boiling hydrolysis, under mobile phase A of 1% HCOOH, B of 1% HCOOH - CH3 CN, v of 0.8 ml/min,A of 530 nm. It was detected by UV-HLPC. [ Result] Sample addition recovery was 75.7% -92.7%. Method precision was 2.7% - 6.3%. There was a linear relationship between the peak area and the concentration at the concentration range of 1 - 200 μg/ml. The minimum detection limit was 0.03 -0.11 mg/kg,and the minimum quantitation limit was 0. 10 -0.35 mg/kg. [ Conclusion] The method was simple,rapid and stable,which could be used as an effective method to determine the anthocyanin in plant samples.

关 键 词：植物样品 花青素 高效液相色谱 

分 类 号：S131[农业科学—农业基础科学]