依托泊苷联合紫杉醇对前列腺癌细胞株生长的协同抑制作用  

Synergistic inhibitory effect of etoposide and taxol on growth of human prostate cancer cell line

作　　者：刘星言[1] 黄国良[1] 邹颖[1] 王建[1] 何志巍[1] 丁聪聪[1] 刘宏[1] 

机构地区：[1]广东医学院,广东省东莞市523808

出　　处：《中华腔镜泌尿外科杂志（电子版）》2013年第4期57-59,共3页Chinese Journal of Endourology(Electronic Edition)

基　　金：国家自然科学基金(81071638);广东省科技计划项目(2011B031700066)

摘　　要：目的研究单独或联合使用依托泊苷(ETOP)和紫杉醇(TAXOL)对前列腺癌细胞株生长和细胞凋亡的影响。方法前列腺癌细胞株经过不同浓度的ETOP和TAXOL单独或联合用药处理后,用MTT法检测细胞抑制率,荧光显微镜观察细胞染色质形态的改变,DNA梯度定性检测细胞凋亡的变化。结果单独利用ETOP或TAXOL和联合用药,均能抑制前列腺癌细胞生长,并呈浓度和时间依赖关系,其中联合用药组抑制率明显高于单独用药组(P<0.05)。ETOP、TAXOL及联合用药组细胞核出现典型的凋亡形态学的改变,细胞数目明显少于对照组,联合用药组细胞数目最少。ETOP、TAXOL及联合用药组处理后的前列腺癌细胞的DNA,经琼脂糖凝胶电泳均出现典型"梯形"DNA条带,联合用药组改变最明显。结论 ETOP和TAXOL均能够抑制前列腺癌细胞生长,联合用药能产生协同抑制作用。Objective To investigate the effects of etoposide（ETOP） and TAXOL on growth and cell apoptosis of human prostate cancer cell line.Methods After prostate cancer cells were cultured with ETOP,TAXOL,or the combination of both chemicals,cell survival was assessed by MTT assay.Cell chromatin morphologic changes were observed under fluorescence microscope,and cell apoptosis were determined by DNA ladder assay.Results ETOP and TAXOL,used alone or in combination,inhibited the growth of prostate cancer cells in a time-and dose-dependent manner.The inhibition was more obvious in the combination group than ETOP group and TAXOL group（P〈0.05）.Typical changes of apoptotic cells in morphology can be observed in ETOP and TAXOL and combination group.The number of cell in combination group is lowest in all three groups.In all groups,a continual diffusive tailed tape and DNA-tailed tape were observed,especially for combination group.Conclusions ETOP and TAXOL can inhibit the growth and induce apoptosis of prostate cancer cells.Furthermore,the combination of ETOP and TAXOL can exert synergistic inhibitory effect on the growth of prostate cancer cells.

关 键 词：依托泊苷 紫杉醇 前列腺癌 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]