Mechanical force-induced DNA damage during AFM single-molecule manipulation  

作　　者：ZHANG Chen DUAN Na DAI Bin ZHANG Yi ZHANG Donghua HU Jun 

机构地区：[1]Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Jiading Campus [2]University of Chinese Academy of Sciences

出　　处：《Nuclear Science and Techniques》2013年第3期66-71,共6页核技术（英文）

基　　金：Supported by National Basic Research Program of China(973 Program Nos.2007CB936000 and 2012CB932600);National Natural Science Foundation of China(No.21073222);Chinese Academy of Sciences(No.KJCX2-EW-N03)

摘　　要：Many environmental factors can cause DNA damage, such as radiation, heat, oxygen free radical, etc., which can induce mutation during DNA replication. Meanwhile, DNA molecules are subjected to various mechanical forces in numerous biological processes. However, it is unknown whether the mechanical force would induce DNA damage and introduce mutation during DNA replication. With the combination of single-molecule manipulation based on atomic force microscopy (AFM), single molecular polymerase chain reaction (SM-PCR) and Sanger's sequencing, we investigated the effect of mechanical force on DNA. The results show that mechanical force can cause DNA damage and induce DNA mutation during amplification.Many environmental factors can cause DNA damage, such as radiation, heat, oxygen free radical, etc., which can induce mutation during DNA replication. Meanwhile, DNA molecules are subjected to various mechanical forces in numerous biological processes. However, it is unknown whether the mechanical force would induce DNA damage and introduce mutation during DNA replication. With the combination of single-molecule manipulation based on atomic force microscopy （AFM）, single molecular polymerase chain reaction （SM-PCR） and Sanger＇s sequencing, we investigated the effect of mechanical force on DNA. The results show that mechanical force can cause DNA damage and induce DNA mutation during amplification.

关 键 词：原子力显微镜 DNA损伤 单分子操纵 机械力 诱导 DNA突变 DNA复制 聚合酶链反应 

分 类 号：Q754[生物学—分子生物学]