乳腺癌干细胞RNA-DCs疫苗诱导特异性细胞毒性T淋巴细胞抗肿瘤免疫应答  

The anti-tumor immune response of specific cytotoxic T lymphocytes induced by breast cancer stem cells RNA-DCs vaccine

作　　者：钟志宏[1] 鄢俊[2] 施华球[2] 

机构地区：[1]赣南医学院教务处,江西省341000 [2]赣南医学院第一附属医院肿瘤科

出　　处：《江苏医药》2013年第13期1489-1491,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(30260039);江西省自然科学基金(2007GZY2599)

摘　　要：目的制备负载乳腺癌干细胞RNA的树突状细胞(DCs)疫苗,研究其诱导的特异性细胞毒性T淋巴细胞(CTLs)的抗肿瘤免疫反应。方法采用细胞毒性试剂盒检测2种乳腺癌干细胞疫苗[(A组,CD44+CD24-+MCF-7-CTLs)、(B组,MCF-7-CTLs)]和DC-CTLs(C组)的体外细胞杀伤能力。取24只小鼠均分为四组:A1组皮下接种活化的A组疫苗+MCF-7乳腺癌细胞;B1组接种活化的B组疫苗+MCF-7乳腺癌细胞;C1组皮下注射活化的DCs-CTLs+MCF-7乳腺癌细胞;D1组单用MCF-7乳腺癌细胞作为对照。分析裸鼠接种后肿瘤在体内的生长情况。结果体外对CD44+CD24-+MCF-7乳腺癌细胞杀伤能力强度:A组>B组>C组(P<0.05)。体外对MCF-7乳腺癌细胞杀伤能力强度:B组>A组>C组(P<0.05)。在体成瘤实验显示,B1组和A1组的成瘤时间分别为第7周和第6周,明显长于D1组(第1周)和C1组(第2周)(P<0.05)。结论 CD44+CD24-乳腺癌干细胞RNA-DCs疫苗诱导的CTLs能够产生特异性免疫应答,从而抑制乳腺癌细胞成瘤。Objective To prepare dendritic cells（DCs） vaccine loaded breast cancer stem cells RNA and explore the anti-tumor immune response of specific cytotoxic T lymphocytes （CTLs）. Methods Killing capacity of breast cancer stem cells vaccines in vitro was detected by cytotoxicity kit in groups of A（treated with CD44＋ CD24 ＋MCF-7-CTLs）, B（treated with MCF 7 CTLs） and C （treated with DC-CTLs）. Twenty-four mice were equally assigned into 4 groups of A1 （subcutaneous injection of activated CD44＋ CD24- ＋ MCF-7-CTLs q- MCF-7 breast cancer cells）, B1 （activated MCF-7-CTLs＋ MCF-7 breast cancer cells）,C1 （activated DC-CTLsq- MCF-7 breast cancer cells） and DI（MCF-7 breast cancer cells alone）. The growth of xenografts was observed. Results In vitro,the killing capacity on CIN4＋CD24 ＋MCF7 breast cancer cells was in an order of group A〉group B〉group C（P〈0. 05） and that on MCF7 breast cancer cells was in an order of group B〉group A 〉 grop C（P〈0. 05）. The time for xenograft formation was 7 weeks for group B1 and 6 weeks for group Al,which was significantly longer than 1 week for group D1 and 2 weeks for group C1 （P〈0. 05）. Conclusion The CTLs induced by CD44＋ CD24 breast cancer stem cells RNA-DCs vaccine can generate a specific immune response, which has an inhibitory effect on tumor formation of breast cancer cells.

关 键 词：乳腺癌 树突状细胞 树突状细胞疫苗 细胞毒性T淋巴细胞 

分 类 号：R737[医药卫生—肿瘤]