钙信号在PAR2激动剂促进SW620细胞增殖中的作用  

Effects of calcium signaling pathway on proliferation of colon cancer SW620 cells induced by PAR2 agonists

作　　者：王静[1] 吴莺[1] 余小燕[1] 蒋双红[1] 胡丽超[2] 周红[2] 

机构地区：[1]江苏大学附属医院消化病科,212013 [2]江苏大学基础医学与医学技术学院

出　　处：《江苏医药》2013年第13期1492-1495,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2010336)

摘　　要：目的探讨钙信号在蛋白酶活化受体2激动剂(PAR2-AP)促进结肠癌SW620细胞增殖中的作用。方法 PAR2-AP 100μmol/L作用于SW620细胞,用fluo-4/AM荧光法测定细胞内Ca2+荧光强度的变化;乙二醇-双-(2-氨基乙醚)四乙酸(EGTA)1mmol/L、毒胡萝卜内酯(TG)1μmol/L、PAR2-AP 100μmol/L预处理细胞后,Western blot检测磷酸化细胞外信号调节激酶1/2(p-ERK1/2)的变化,MTT法检测SW620细胞增殖活力的变化,流式细胞术检测细胞周期的变化。结果 PAR2-AP刺激SW620细胞后,Ca2+荧光强度短暂升高后减低;EGTA和TG预处理细胞能明显干预PAR2-AP对p-ERK1/2的升高作用及其促细胞增殖作用。结论 PAR2-AP活化PAR2受体后经钙信号通路影响p-ERK1/2表达,促进SW620细胞的增殖。Objective To elucidate the effects of calcium signaling on the proliferation of colon cancer SW620 cells induced by protease-activated receptor 2 agonists （PAR2-AP）. Methods The SW620 cells were treated with PAR2-AP and the changes of intracellular Ca2＋ concentration （[Ca2＋]i） were detected with fluo-4/AM using confocal laser scanning microscopy. After the cells were pretreated with ethylene glycol tetraacetic acid （EGTA）, thapsigargin and PAR2-AP, the expression of phosphorylated extracellular signal-regulated kinase 1/2 （p-ERK1/2）, the proliferative potential of SW620 cells and the changes of cell cycle distribution were detected by Western blot, MTT and flow cytometry, respectively. Results [Ca2＋ ]i was transiently elevated in SW620 cells after stimulated by PAR2-AP. The activation of p-ERK1/2 and the proliferation of SW620 cells induced by PAR2-AP could be inhibited by EGTA and thapsigargin pretreatment. Conclusion PAR2-dependent calcium signaling pathway is critical for activation of p-ERK1/2 and proliferation of SW620 cells induced by PAR2-AP.

关 键 词：结肠癌 蛋白酶激活受体2 钙信号 细胞外信号调节激酶 

分 类 号：R735[医药卫生—肿瘤]