生长分化因子-9在人膀胱癌组织中的表达及其临床意义  

Expression and its clinical significance of growth differentiation factor-9 in bladder cancer

作　　者：符骏[1] 杜鹏[2,3,4] 杨勇[2,3,4] 

机构地区：[1]北京京煤集团总医院泌尿外科,北京102300 [2]北京大学肿瘤医院 [3]北京市肿瘤防治研究所泌尿外科 恶性肿瘤发病机制及转化研究教育部重点实验室 [4]首都医科大学附属北京医院泌尿外科

出　　处：《临床泌尿外科杂志》2013年第7期509-512,共4页Journal of Clinical Urology

基　　金：首都医科大学基础与临床合作基金(编号12JL41)

摘　　要：目的:探讨生长分化因子-9(growth differentiation factor-9,GDF-9)在人膀胱癌组织中的表达及其与膀胱癌病理分级和临床分期的关系。方法:运用免疫组化技术检测GDF-9在50例不同分期分级的膀胱癌组织和15例正常膀胱组织之间的蛋白表达情况。结果:①正常人膀胱组织中GDF-9呈高表达,且GDF-9蛋白主要定位于膀胱移行上皮细胞的胞浆中。而膀胱癌组织中GDF-9呈低表达或不表达。②GDF-9阳性表达率在膀胱癌病理分级间分别:Ⅰ级47.6%(10/21),Ⅱ级26.7%(4/15),Ⅲ级7.1%(1/14),差异具有统计学意义(P<0.05)。GDF-9阳性表达率在膀胱癌临床分期间分别:Ta～T1期40.6%(13/32),T2～T3期11.1%(2/18),差异具有统计学意义(P<0.05)。结论:GDF-9在膀胱癌组织中低表达或不表达,且与膀胱癌病理分级和临床分期呈负相关。GDF-9可能是一个潜在的人膀胱肿瘤抑癌基因。Objective： To investigate the expression of growth differentiation factor-9 （GDF-9） in bladder transitional cell carcinoma （BTCC） and its clinical significance in the malignancy of BTCC. Method： Immunohistochemical methods was used to detect GDF-9 protein in 50 cases of BTCC and 15 cases of normal bladder muscosa served as the controls. Result： In normal bladder tissues, stronger staining of GDF 9 was seen in transitional cells, both in the cytoplasm and in the nucleus. In contrast, the staining of GDF-9 was notably weak or absent in cancer cells of tumour tissues. The positive expression rate of GDF9 in Grade I , II and III according to pathological grade of BTCC was 47. 6% （10/21）, 26. 7G （4/15） and 7. 1% （1/14）, respectively. There were significant differences among the three pathological grades. The positive expression rate of GDF9 in Stage Ta-T1 and T2-T3 according to clinical stages of BTCC was 40.6G （13/32） and 11.1%（2/18）, respectively. There were significant differences between the two clinical stages. Conclusion： GDF-9 is expressed at lower levels in human bladder cancer tissues compared with normal transitional cells of the bladder. GDF-9 levels are inversely correlated with the path ological grade and clinical stages of bladder cancer. It suggests that GDF-9 is a potential turnout suppressor in human bladder cancer.

关 键 词：膀胱肿瘤 生长分化因子-9 

分 类 号：R737.14[医药卫生—肿瘤]