神经节苷脂对脑缺血再灌注大鼠海马Fas表达的影响  

Effects of Monosialoganglioside on the Expression of Fas in Hippocampus of Cerebral Ischemia-Reperfusion Rats

作　　者：杜江[1] 铁欣昕[1] 欧绍武[1] 

机构地区：[1]中国医科大学附属第一医院神经外科,沈阳110001

出　　处：《天津医药》2013年第7期686-688,共3页Tianjin Medical Journal

摘　　要：目的探讨单唾液酸神经节苷脂(GM)1对脑缺血再灌注大鼠海马Fas表达的影响。方法 72只SD大鼠随机分为假手术组(8只)、GM1治疗组(32只)、缺血再灌注组(32只)。GM1治疗组和缺血再灌注组依据缺血后再灌注的不同时间点再分为6h、12h、24h、3d共4个小组。应用免疫组织化学方法和Western blot方法检测各组大鼠海马Fas蛋白的表达。结果免疫组织化学方法结果显示假手术组大鼠海马有一定量的Fas蛋白表达(0.17±0.02),而缺血再灌注组大鼠海马的Fas蛋白阳性表达显著升高,各时点分别为0.42±0.11、0.51±0.13、0.55±0.13及0.62±0.15,3d时表达最高;与相同时间点的缺血再灌注组比较,GM1治疗组的Fas蛋白阳性表达则明显降低,各时点分别为0.29±0.09、0.34±0.11、0.37±0.12、0.43±0.12(均P<0.05)。Western blot结果与此结果类似。结论 GM1很可能通过减少Fas蛋白的表达参与脑缺血再灌注损伤的神经保护作用。Objective To study the expression of Fas in hippocampus of cerebral ischemia-reperfusion rats after treatment with monosialoganglioside （GM1）. Methods Seventy-two SD rats were randomly divided into sham group （n=8）, GMI treatment group （n=32） and iscbemia-reperfusion （I/R） group （n=32）. According to the different time points （6 h, 12 h, 24 h and 3 d） of I/R, there were four subgroups in GM1 and I/R groups respectively. The expression of Fas in hippocampus was examined by immunochemistry and Western blot methods in all groups. Results Results of immunochemistry method showed that there was a little expression of Fas （the mean optical density was 0.17±0.02） in hippocampus of sham group, and the positive expression of Fas at different time points were increased significantly after reperfusion. The mean values of optical density at different time points were 0.42±0.11, 0.51±0.13, 0.55±0.13 and 0.62±0.15 in I/R group, which reached to the peak at 3 d . The positive expression of Fas at different time points were significantly decreased in GM1 group （0.29±0.09, 0.34±0.11, 0.37±0.12 and 0.43±0.12） than that of I/R group （P 〈 0.05）. Conclusion Monosialoganglioside participated in the pathogenesis of cerebral ischemia-reperfusion injury by down-regulating the expression of Fas.

关 键 词：再灌注损伤 G(M1)神经节苷脂 海马 Fas配体蛋白质 大鼠 Sprague-Dawley 

分 类 号：R743.3[医药卫生—神经病学与精神病学]