不同注射VEGF途径对局灶性脑缺血再灌注大鼠侧支循环形成的影响  

Collateral circulation in focal cerebral ischemia rats after being injected vascular endothelial growth factor by three different injection ways

作　　者：宋学萍[1] 孙盛同 郭阳[2] 邓月香[1] 周俊球 王艳[1] 彭勃[1] 

机构地区：[1]广州市白云区人民医院神经内科,510500 [2]南方医科大学珠江医院神经内科,广州510282

出　　处：《中华神经医学杂志》2013年第7期661-664,共4页Chinese Journal of Neuromedicine

基　　金：广州市白云区2012年科技计划（2012-KZ-131

摘　　要：目的探讨鞘内注射、静脉注射与颅内注射3种不同的血管内皮生长因子（VEGF）给药途径对局灶性脑缺血再灌注大鼠侧支循环形成的影响。方法健康雄性SD大鼠60只采用随机数字表法分为鞘内注射组、颅内注射组、静脉注射组、手术组和假手术组，每组12只。前4组大鼠采用改进的线栓法制作局灶性脑缺血再灌注损伤模型，其中前3组大鼠造模后即刻、24h、48h分别于鞘内、颅内、静脉注射10ngVEGF。造模后12h、3d对大鼠进行神经功能评分，模型后3、7、14d免疫组化染色检测各组大鼠脑梗死半影区血管内皮细胞VEGF及F8因子的表达。结果免疫组化染色显示鞘内注射组、颅内注射组、静脉注射组、手术组大鼠脑梗死半影区VEGF在再灌注后3d、7d、14d依次增多，差异有统计学意义（o〈0．051；造模后3、7、14d鞘内注射组、颅内注射组大鼠血管内皮细胞VEGF表达水平高于静脉注射组、手术组．而静脉注射组高于手术组，差异均有统计学意义（p〈0．05）。鞘内注射组、颅内注射组、静脉注射、手术组大鼠梗死半影区F8因子在再灌注后3d、7d、14d依次增多，差异有统计学意义（p〈0．05）；造模后3d、7d鞘内注射、颅内注射组、静脉注射组、手术组大鼠脑梗死半影区F8因子的表达差异无统计学意义（P〉O．05）；造模后14d鞘内注射组、颅内注射组大鼠F8因子的表达高于静脉注射组、手术组．而静脉注射组高于手术组，差异有统计学意义（p〈0．05）。结论3种不同vEGF给药途径都可以促进局灶性脑缺血再灌注大鼠侧支循环形成．但鞘内注射与颅内注射这两种途径的干预效果优于静脉注射。Objective To study the collateral circulation in focal cerebral ischemia rats after being injected vascular endothelial growth factor （VEGF） by three different injection ways （intrathecal injection, intracranincal injection and intravenous injection）. Methods Sixty healthy male SD rats were randomly divided into five groups： intrathecal injection （IT） group, intracranincal injection （IC） group, intravenous injection （IV） group, operation （OP） group and sham operated （SH） group （n=12）. Middle cerebral artery occlusion （MCAO） was performed to induce focal cerebral ischemia models in rats of the four groups, except the sham-operated group; 10 ng VEGF was given to the injection groups right after the inducement, 24 and 48 h after the inducement. The neurological scale was performed in all the rats 12 h and 3 d after the inducement. The expressions of VEGF and F8 in the periphery of infarcts were detected in the five groups 3, 7 and 14 d after ischemia reperfusion. Results The VEGF expressions in the four groups, expected the sham-operated group, increased gradually 3, 7 and 14 d after ischemia reperfusion, with significant difference between each two time points （P〈0.05）. The VEGF expressions of IT and IC groups were higher than that in the IV and OP groups （P〈0.05）, and that of 1V group wassignificantly higher than that of OP group （P〈0.05）. The F8 expression in the four groups, expected the sham-operated group, increased gradually 3, 7 and 14 d after ischemia reperfusion, with significant difference between each two time points （P〈0.05）. The F8 expression of IT and IC groups was obviously higher than that of the IV and OP groups 14 d after reperfusion （P〈0.05）, and that of 1V group was significantly higher than that of OP group （P〈0.05）. Conclusion All the three different injection ways can improve the formation of collateral circulation in MACO rats, but intrathecal and intracranincal injections are better than intravenous injection.

关 键 词：局灶性脑缺血 血管内皮生长因子 侧支循环 鞘内注射 颅内注射 静 脉注射 

分 类 号：R743.3[医药卫生—神经病学与精神病学]