IL-27在多发性骨髓瘤患者及骨髓瘤细胞系中的表达  

Expression of IL-27 in multiple myeloma and its cell lines

作　　者：夏天[1] 李建平[1] 李文倩[1] 王莉[1] 郝星[1] 孙方方[1] 戴昕[1] 阿祥仁[2] 彭海[2] 宋玉君[2] 冯建明[1] 

机构地区：[1]青海省人民医院血液科,西宁810007 [2]青海省人民医院检验科,西宁810007

出　　处：《中华血液学杂志》2013年第7期626-628,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金（81160071）

摘　　要：目的研究IL-27在多发性骨髓瘤（MM）患者及骨髓瘤细胞系U266、RPM18226培养上清中的水平及其意义。方法采用ELISA法检测MM患者及正常对照者血浆中IL-27和IL－6表达水平；采用ELISA法检测U266、RPM18226细胞培养上清中IL-27表达水平；实时定量RT—PCR方法检测MM患者外周血单个核细胞IL-27 mRNA表达水平。结果MM患者血浆IL-27水平为（61．82±8．01）ng／L，明显高于正常对照组[（8．29±4．41）ng／L]（P〈0．05）；MM患者血浆IL-6水平为（45．62±1．24）ng／L，也明显高于正常对照组[（2．27±0．18）ng／L]（P〈0．05）；U266、RPM18226细胞培养上清中IL-27水平分别为（50．06±5．72）和（335．47±41．88）ng／L。RT—PCR结果证实MM患者外周血单个核细胞IL-27 mRNA相对表达水平较正常对照组明显升高，差异有统计学意义（P〈0．05）。结论MM患者高表达IL-27，骨髓瘤细胞系U266、RPM18226自分泌IL-27，IL-27可能参与MM的发病机制。Objective To study the expression and significance of IL-27 in patients with multiple myeloma （MM） and in the supernatant of MM cell lines U266 and RPMI8226 cells culture medium. Methods A total of 20 MM patients and 20 controls were enrolled in this study. The expressions of IL-27 and IL- 6 in MM patient＇ s blood plasma, and the expression of IL- 27 in U266 and RPMI8226 culture supernatant were measured by enzyme-linked immunosorbent assay （EL1SA）. The mRNA expression of IL- 27 in mononuclear cells of MM patients＇ peripheral blood was measured by real-time quantitative polymerase chain reaction （RT-PCR）. Results The levels of IL-27 in plasma of MM patients and normal controls were （61.82±8.01） ng/L and （8.29±4.41） ng/L （P〈0.05）, and those of IL-6 were （45.62±1.24） ng/L and （2.27 ±0.18） ng/L （P〈0.05）, respectively. The levels of IL-27 in U266 and RPMI8226 culture supernatant were （50.06±5.72） ng/L and （335.47±41.88） ng/L. RT-PCR revealed that the levels of IL-27 mRNA were up-regulated in MM patients compared to controls. Conclusion High expression of IL-27 is observed in MM patients and MM cell lines U266 and RPMI8226 cells. IL-27 may play a important role in pathogenesis of MM.

关 键 词：白细胞介素27 白细胞介素6 多发性骨髓瘤 

分 类 号：R733.3[医药卫生—肿瘤]