牙龈上皮细胞中人β2——防御素mRNA的表达  

Expression of Human β-defensin-2 in Gingival Epithelial Cell

作　　者：魏洪涛[1] 罗云纲[2] 王国珍[1] 李美华[2] 

机构地区：[1]吉林大学中日联谊医院口腔科,吉林长春130031 [2]吉林大学第二医院口腔科

出　　处：《口腔医学研究》2013年第7期604-606,共3页Journal of Oral Science Research

基　　金：吉林省科技发展计划项目(编号:200705313)

摘　　要：目的:检测人β防御素(humanβdefensin,hBD)2mRNA在牙龈增生病人和正常人的牙龈上皮细胞中的表达,分析hBD-2mRNA在牙龈组织天然免疫中的作用。方法:应用逆转录一聚合酶链反应技术(reverse tran-scriptase-polymerase chain reaction,RT-PCR)检测12例牙龈增生病人和10例正常牙龈上皮细胞中hBD-2mRNA的表达。结果:hBD-2mRNA在l2例牙龈增生和l0例正常对照牙龈上皮细胞中均有表达,且两组间差异有统计学意义(P<0.05)。结论:牙龈上皮细胞中hBD-2在天然免疫中发挥重要作用,hBD-2的表达增加可能与牙龈增生中的细胞因子有关。Objective： To examine the expression of human β-defensin-2（hBD-2） in gingival epithelial cell of normal subjects and patients with gingival hyperplasia,and to investigate the role of β-defensin 2 in the innate immunity.Methods： Gingival tissues were obtained from 10 normal subjects and from 12 patients undergoing surgery for gingival hyperplasia.Isolated epithelial cells were used for total RNA isolation by reverse transcriptase-polymerase chain reaction RT-PCR）.Results： β-defensin-2 mRNA was detected in the gingival epithelium of patients with gingival hyperplasia normal gingival tissue.Its expression level was significantly higher in diseased epithelium than in normal turbinate epithelium（P0.05）.Conclusion： These results suggest that beta-defensin 2 may play a constitutive role in gingival defenses.It may be induced in response to local infection or inflammation.

关 键 词：人β-防御素2 牙龈增生 上皮细胞 

分 类 号：R781.4[医药卫生—口腔医学]