MMP-2的抑制作用在治疗大鼠口腔溃疡中的实验研究  

Effects of Inhibiting the Activity of MMP-2 on Oral Ulcers in Rats

作　　者：刘振宇[1] 张立田[1] 蔡攀[1] 李正强[1] 陈黄琴[1] 

机构地区：[1]湖北科技学院五官医学院,湖北咸宁437100

出　　处：《口腔医学研究》2013年第7期613-616,共4页Journal of Oral Science Research

摘　　要：目的:探究抑制基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)的活性对于实验性大鼠口腔溃疡的治疗效果。方法:采用浓度为75%醋酸溶液制备大鼠口腔溃疡模型,待造模成功后随机分组,各组每天给予不同浓度的强力霉素进行干预(对照组不用强力霉素)。分别在1、3、5、7d观察大鼠的全身状况、肉眼下黏膜损伤情况、光镜下溃疡黏膜病理改变情况以及组织切片中Masson胶原三色染色、MMP-2的表达水平。结果:同一时间点,强力霉素干预组炎性渗出少,胶原Masson染色颜色较浅。干预组MMP-2的累积光密度值(integrated opticaldensity,IOD)显著低于对照组(P<0.01),其中大剂量组IOD值为2186.56±926.41,小剂量组为5416.12±941.56,差异具有显著性(P<0.01)。结论:MMP-2的活性抑制具有促进口腔溃疡愈合的作用,并在一定浓度范围内,随药物浓度升高,溃疡的愈合效果越好。Objective： To explore the therapeutic effects of inhibiting the activity of MMP-2（matrix metalloproteinase-2） on the experimental rats＇ oral ulcers.Methods： The oral ulcer models of rats were induced by 75% acetic acid and SD rats were randomly allocated to Doxycycline intervention groups and control group.1,3,5,7days after Doxycycline intervention,the general conditions of the rats,the damage of oral mucosa under the naked eye,the mucosal pathological changes,Masson collagen trichrome staining and expression levels of MMP-2 under the optic microscope were observed.Results： At the same time point,Doxycycline could reduce inflammatory exudation and lighten Masson collagen staining.The positive integrated optic density（IOD） of the cells in the intervention group was remarkably lower than the control group（P0.01）.The IOD of the higher dose and lower dose group was 2186.56±926.41 and 5416.12±941.56,respectively,and there was statistical difference between these two groups（P0.01）.Conclusion： MMP-2 activity inhibition can improve the healing of oral ulcer in concentration-dependent manner.Within a limited range of concentration,the higher the concentrations are,the more obvious effects are.

关 键 词：基质金属蛋白酶 口腔溃疡 强力霉素 

分 类 号：R781.5[医药卫生—口腔医学]