卵巢上皮性癌中肌切蛋白的表达及其临床意义  

The Expression of Scinderin in Epithelial Ovarian Cancer and the Clinicopathologic Significance

作　　者：吴姗[1] 马晶晶[1] 姜旖[1] 马小玲[1] 范文[1] 程文俊[1] 

机构地区：[1]南京医科大学第一附属医院,江苏南京210029

出　　处：《实用妇产科杂志》2013年第7期522-525,共4页Journal of Practical Obstetrics and Gynecology

基　　金：国家自然科学基金项目(项目编号:81272871);江苏省科技厅自然科学基金项目(项目编号:BK2010576);江苏省六大人才高峰项目(项目编号:303070774IB09);江苏省医学重点人才项目[苏卫科教(2011)15号]

摘　　要：目的:探讨肌切蛋白(Scinderin)在卵巢上皮性癌中的表达及其临床意义。方法:运用实时荧光定量聚合酶链式反应(PCR)法和蛋白质印迹(Western blot)法分别检测34例卵巢上皮性癌、21例卵巢良性肿瘤和20例正常卵巢组织中肌切蛋白mRNA和蛋白的表达情况,并进行临床资料统计,分析肌切蛋白与卵巢上皮性癌临床病理参数间的关系。结果:卵巢上皮性癌组中肌切蛋白mRNA及蛋白表达量均显著高于良性卵巢肿瘤组和正常卵巢组(P<0.01),而在正常卵巢组与良性卵巢肿瘤组中,肌切蛋白mRNA及蛋白表达量差异无统计学意义(P>0.05)。肌切蛋白mRNA和蛋白表达与卵巢上皮性癌病理分级、淋巴结转移有关(P<0.01;P<0.05),而与患者年龄、肿瘤临床分期无相关性(P>0.05)。结论:肌切蛋白在卵巢上皮性癌中表达上调,且在低分化卵巢上皮性癌中表达高于中-高分化卵巢上皮性癌,有淋巴结转移者高于无淋巴结转移者,提示肌切蛋白与卵巢上皮性癌患者的不良预后相关,可作为判断卵巢上皮性癌预后的生物学标志物。Objective：To investigate the expression and clinical significance of Scinderin gene in epithelial ovarian cancer. Methods：The expression of Scinderin in 34 cases of epithelial ovarian Cancers,21 cases of benign ovarian epithelial tumors and 20 cases of normal ovarian tissues were examined by real-time PCR and Western blot. The correlation between the expression of Scinderin and clinicopathologic features was further analyzed. Results： Expression level of Scinderin in normal ovarian tissues and benign ovarian epithelial tumors were both significantly lower than in epithelial ovarian cancers （ P 〈 0.01 ）. The expression of Scind- erin has no significant difference in normal ovarian and benign ovarian epithelial tumors（ P 〉0.05）. The ex- pression of Scinderin was correlation with histological grade and lymphatic metastasis（ P 〈 0.01, P 〈 0. 05）, but not with age and FIGO stages （P 〉 0.05）. Conclusions：Expression level of Scinderin was significantly higher in epithelial ovarian cancer and has a significant correlation with the poor prognosis,so it could be a bi- ological marker for ovarian cancer.

关 键 词：卵巢上皮性癌 肌切蛋白 实时荧光定量聚合酶链式反应 蛋白质印迹 

分 类 号：R737.31[医药卫生—肿瘤]