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作 者:林粱[1] 杨定华[1] 黄毓[1] 李湘竑[1] 张庆玲[2] 刘萧[1] 梁鉴坤[1] 钟克波[1] 何华[1]
机构地区:[1]南方医科大学南方医院肝胆外科,广州510515 [2]南方医科大学南方医院病理科,广州510515
出 处:《中华医学杂志》2013年第28期2186-2190,共5页National Medical Journal of China
基 金:广东省教育厅人才引进基金
摘 要:目的观察吲哚胺2,3双加氧酶(IDO)在肝细胞肝癌(HCC)中的表达,分析其表达与临床病理参数的关系。方法采用逆转录.聚合酶链反应(RT—PCR)、荧光定量PCR、免疫组化、免疫荧光研究IDO在HCC中的表达。结果IDOmRNA在癌组织中的表达比相应的非癌组织明显增高(2=1.71,P=0.001),ACT值在癌组织中为6.40±2.21,非癌组织中7.17±1.90,正常肝脏组织中7.61±0.93。IDO蛋白主要在癌边缘及癌组织的胞质中表达,而在非癌及肝脏良性病变的正常肝脏组织内则不表达。IDO表达的强弱与术后复发及生存时间、是否发生转移、TNM分期有关(P〈0.05);与肝硬化、AFP、病理分化、巴塞罗那分期、性别、年龄、乙肝表面抗原、肿瘤大小及数目无关(P〉0.05)。结论IDO的高表达可能会影响HCC患者的愈后。Objective To explore the expressions of indoleamine 2, 3-dioxygenase (IDO) in hepatocellular carcinoma and analyze its relationship with clinicopathological parameters. Methods Quantitative real-time polymerase chain reaction ( PCR ) , fluorogenic quantitative PCR, immunohistochemical and immunofluorescence were used to detect the expression of indoleamine 2, 3- dioxygenase in hepatocellular carcinoma. Results The IDO mRNA expression in cancerous tissues increased markedly than that in the corresponding non-cancerous tissues(2-acT = 1. 71, P = 0. 00l ). The immunohistochemical and immunofluorescence results showed that IDO protein was expressed in cytoplasm of hepatocellular carcinoma and tumor-surrounding tissues. But there was no expression in normal liver tissues from benign hepatic lesions and corresponding non-cancerous tissues. An over-expression of IDO protein was detected in 43 patients(48.3% ) , a low expression in 25 (28.1%) and no expression in 21 (23.6%). Relationship between IDO expression and ciinicopathological parameters: an over-expression of IDO in HCC was associated with recurrence, survival time, metastasis and TNM stage ( P 〈 0. 05 ), but not associated with patient's cirrhosis, AFP level, histological differentiation type, Barcelona clinic liver cancer stage, gender, age, HbsAg positivity, number of tumors and tumor size ( P 〉 0. 05 ). Conclusion An over- expression of IDO in HCC patients may affect patient prognosis.
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