制备细菌类单细胞生物扫描电镜样品方法的改进  

The improvement of bacteria sample preparation method for scanning electron microscope observation

作　　者：梁静南[1] 刘一苇[1] 谢家仪[1] 

机构地区：[1]中国科学院微生物研究所大型仪器中心,北京100101

出　　处：《电子显微学报》2013年第3期276-278,共3页Journal of Chinese Electron Microscopy Society

摘　　要：常规扫描电镜生物样品制备过程中要经过多次漂洗和脱水,细菌类样品亦或单细胞样品体积微小,制备的每步环节都需要离心收集。多次离心容易导致样品丢失,尤其是高通量的样品制备时,丢失更为严重。本文实验结果表明:在3次漂洗固定液之后用滤纸包收集样品,由于乙醇的渗透速度快,滤纸并不会对乙醇的脱水造成太大的影响。使用改进后的方法,不但能保证观察所需的样品数量,而且也节省了操作时间。The conventional SEM sample preparation needs multiple steps including fixing, rinsing, and dehydrating. Because the volume of microbiology sample or unicellular sample is so small, so each step of preparation needs centrifugal collection. However, multiple centrifuging may cause loss of the sample. In this study, filter paper pack is used to collect sample. This method significantly improves the efficacy of the sample preparation and enhances the observing effect of the sample.

关 键 词：样品制备 改进 扫描电镜 

分 类 号：Q254[生物学—细胞生物学]