癫痫持续状态大鼠海马线粒体分裂、融合的变化  

Alteration of mitochondrial fission and fusion in hippocampus of rats with status epilepticus

作　　者：邱小雪[1] 曹丽丽[1] 杨雪[1] 迟兆富[1] 

机构地区：[1]山东大学齐鲁医院神经内科,济南250012

出　　处：《山东大学学报（医学版）》2013年第7期15-19,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81100971;81071041);山东省自然科学基金(Y2007C117)

摘　　要：目的探讨癫痫持续状态大鼠海马线粒体分裂、融合的变化。方法将50只Wistar大鼠随机分为正常对照组和癫痫组,癫痫组分为癫痫持续状态后4、8、24和72 h组,观察癫痫持续状态后不同时间点大鼠海马线粒体分裂、融合的变化。Western blotting、PCR分析海马线粒体中Drp1、hFis1、Mfn1、Opa1的表达;免疫组化分析海马CA3区神经细胞Drp1、Opa1的表达。结果 Drp1、hFis1在癫痫持续状态后4 h时开始升高,24 h达高峰,72 h仍处于较高水平;Mfn1、Opa1在癫痫持续状态后4 h时出现短暂的升高,随即表达下降,并于24 h时达最低水平。癫痫持续状态后24 h时,大鼠海马CA3区Drp1阳性神经元数目显著高于对照组(P<0.05),Opa1阳性神经元数目显著低于对照组(P<0.05)。结论癫痫持续状态大鼠海马线粒体分裂、融合失衡,主要表现为线粒体分裂增强、线粒体融合抑制。Objective To investigate the alteration of mitochondrial fission and fusion in hippocampus of rats with sta- tus epilepticus （SE）. Methods Male Wistar rats were randomly divided into five groups ： normal control group and ep- ileptic group （4, 8, 24 and 72 h after SE）. The alteration of mitochondrial fission and fusion in hippocampus of rats were evaluated at different time points after SE. Western blotting and PCR were used to estimate the expression of dy- namin related proteinl （ Drpl ）, human mitochondrial fission proteinl （ hFisl ）, mitofusinl （ Mfnl ） and optic atrophyl gene proteinl （ Opal ）. Immunohistochemistry was used to estimate the expressions of Drpl and Opal in neurons of hippocampal CA3 region 24 h after SE. Results Mitochondrial fission proteins Drpl and hFisl began to increase 4 h af- ter SE, with a peak at 24 h and still elevated 72 h after SE. Whereas mitochondrial fusion proteins Mfnl and Opal in- creased transiently at 4 h after SE and decreased sharply thereafter, falling into the bottom at 24 h. The number of neu- rons expressed Drpl at 24 h after SE was significantly higher than that in normal control group （ P 〈 0.05 ）, while the number of neurons expressed Opal was significantly lower than that in normal control group（ P 〈 0.05 ）. Conclusion SE leads to imbalanced mitochondrial fission and fusion, shown by the enhanced mitochondrial fission and the declined mitochondrial fusion.

关 键 词：癫痫持续状态 海马 线粒体 分裂 融合 

分 类 号：R742.1[医药卫生—神经病学与精神病学]