贵州省神经管畸形影响因素的病例对照研究  

A case-control study on the risk factors of neural tube defects in Guizhou province of China

作　　者：何俊峰[1] 刘俊[2] 王伟人 张青峰[1] 杨琼芬[1] 李光友[1] 周林[1] 李志梅[1] 李佩珍[1] 

机构地区：[1]遵义医学院珠海校区预防医学教研室,广东珠海519041 [2]遵义医学院营养与食品卫生教研室,贵州遵义563003 [3]贵州省疾病控制中心妇幼保健所,贵阳550004

出　　处：《山东大学学报（医学版）》2013年第7期50-53,57,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81060245);贵州省科学技术基金(黔科合J字2008-2316)

摘　　要：目的探讨贵州省神经管畸形(NTDs)高发的危险因素,揭示多因素在NTDs发生中的作用。方法采用1∶1匹配的病例对照研究,对63例神经管缺陷患儿的母亲和63例正常儿的母亲进行问卷调查和血样检查,采用PCR-RFLP方法检测脂肪酸结合蛋白2基因(FABP2)A54T、脂联素基因(APM1)T45G和解偶联蛋白2基因(UCP2)A55V的多态性。运用SPSS 16.0软件进行单因素和多因素统计分析。结果单因素分析发现,FABP2A54T基因型频率分布在病例组和对照组中的差异有统计学意义(P<0.05),APM1 T45G基因型和UCP2 A55V基因型频率分布在两组间的差异无统计学意义(P>0.05);多因素条件Logistic回归分析结果显示,妊娠剧吐(OR=3.56,95%CI:1.17～10.87)、被动吸烟(OR=3.36,95%CI:1.17～9.71)、食用发芽土豆(OR=5.80,95%CI:1.17～28.78)和FABP2 A54T基因多态性(OR=2.41,95%CI:1.13～5.12)为NTDs的危险因素。结论FABP2 A54T基因多态性、母亲妊娠剧吐史、食用发芽土豆和被动吸烟可能是影响贵州省NTDs发生的主要危险因素。Objective To find out some susceptible risk factors and explore the roles of multi-factors in the high inci- dence of neural tube defects （NTDs） in Guizhou province of China. Methods A 1 ： 1 case-control study was conducted on 63 cases who had NTDs infants and 63 cases who had healthy infants. PCR-RFLP was used to detect the polymor- phisms of FABP2 A54T, APM1 T45G, and UCP2 A55V. The data were analyzed by SPSS 16.0 statistical software. Results The distributions of FABP2 A54T gene between the NTDs and control groups had significant differences （ P 〈 0.05 ）, but the distributions of APM1 T45G and UCP2 A55V genes between the two groups had no significant differ- ences （ P 〉 0.05 ）. Multivariate conditional Logistic regression analysis showed that the following four factors were cor- related with NTDs： vomiting during pregnancy （OR --3.56, 95% CI. 1. 17-10.87 ）, passive smoking （OR = 3.36, 95%CI： 1. 17-9.71 ）, germinated potato intake （OR = 5. 80, 95% CI： 1. 17-28. 78）, and FABP A54T mutation （ OR = 2.41, 95% CI. 1.13-5.13 ）. Conclusion The results suggested that the FABP2 A54T polymorphism, vomiting during pregnancy, germinated potato intake and passive smoking are associated with NTDs.

关 键 词：神经管畸形 脂肪酸结合蛋白2基因 脂联素基因 解偶联蛋白2基因 基因多态性 

分 类 号：R181.26[医药卫生—流行病学] R722.1[医药卫生—公共卫生与预防医学]