肝癌中Tiam-1和HPA-1的表达及其与侵袭转移的关系  

Expression levels of Tiam-1 and HPA-1 and their relationship with the invasion and metastasis of hepatocellular carcinoma

作　　者：王贺[1] 孙风波 张凤娟[3] 亓玉琴[4] 

机构地区：[1]青岛市传染病医院肝病科,山东省青岛市266033 [2]青岛市海慈医疗集团普外科 [3]青岛大学医学院附属医院黄岛分院感染科 [4]青岛大学医学院附属青岛市市立医院消化内科

出　　处：《中国肿瘤临床》2013年第13期770-774,共5页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨Tiam-1和HPA-1基因在肝癌中的定量表达及其与肝癌侵袭转移的关系。方法:选取2009年5月至2012年1月行手术治疗的肝癌患者65例。采用实时荧光定量逆转录聚合酶链反应(RT-PCR)方法检测Tiam-1 mRNA及HPA-1mRNA在原发性肝细胞肝癌、配对癌旁组织(距癌2 cm)及正常肝脏组织中(距癌>5 cm)的表达水平,并分析两者与肝癌临床病理参数的关系。结果:肝癌组织中Tiam-1 mRNA的表达水平(43.83±11.62)显著高于癌旁组织(14.82±8.16)和正常肝脏组织(6.02±5.36)(均P<0.05),癌旁组织中Tiam-1 mRNA的表达水平高于正常肝脏组织(P<0.05)。肝癌组织中HPA-1 mRNA的表达水平(35.28±11.81)显著高于癌旁组织(12.94±6.25)和正常肝脏组织(4.17±3.49)(均P<0.05),癌旁组织中HPA-1 mRNA的表达水平高于正常肝脏组织(P<0.05)。Tiam-1及HPA-1的表达增强与肿瘤的分化程度、浸润深度、淋巴结转移、血行转移及TNM分期显著相关(均P<0.05)。Spearman等级相关分析证明了Tiam-1和HPA-1的表达呈明显正相关(OR=0.523,P<0.05)。结论:Tiam-1mRNA和HPA-1 mRNA在肝癌组织中呈高表达,且两者的表达增强可促进肝癌的侵袭转移。联合检测Tiam-1及HPA-1对于指导肝癌临床治疗及判断预后有重要价值。Objective： Invasion and metastasis are the main causes of carcinoma mortality; hence, the timely blocking of the invasion and metastasis of carcinoma has become a research hotspot. The present study aims to investigate the expression levels of Tiam-1 mRNA and HPA-1 mRNA and their correlation with the invasion and metastasis of hepatocellular carcinoma. Methods： From May 2009 to Jan 2012, 65 hepatocellular carcinoma patients admitted consecutively in our hospital for surgical treatment were included in this study. Real-time fluorescent quantitative reverse transcriptase polymerase chain reaction （RT-PCR） was used to investigate the expression levels of Tiam-1 mRNA and HPA-1 mRNA in hepatocellular carcinoma, paired adjacent hepatocellular carcinoma （2 cm from the carcinoma）, and surgical marginal normal hepato mucosa tissues （5 cm from the carcinoma）. RT-PCR was also used to analyze their correlation with the clinicopathological characteristics of hepatocellular carcinoma. Results： The expression level of HPA-1 mRNA was significantly higher in hepatocellular carcinoma （43.83±11.62） than in paired adjacent hepatocellular carcinoma （14.82±8.16） and normal hepato mucosa tissues （6.02±5.36） （P〈0.001）. The expression level of HPA-1 mRNA was higher in paired adjacent hepatocellular carcinoma than in normal hepato mucosa tissues （P〈0.05）. The expression of Tiam-1 mRNA was higher in hepatocellular carcinoma （35.28±11.81） than in paired adjacent hepatocellular carcinoma （12.94±6.25） and normal hepato mucosa tissues （4.17±3.49） （P〈0.05）. The expression level of Tiam-1 mRNA was higher in paired adjacent hepatocellular carcinoma than in normal hepato mucosa tissues （P〈0.05）. The expression levels of Tiam-1 mRNA and HPA-1 mRNA were closely associated with the degree of differentiation, depth of infiltration, lymph node metastasis, vessel metastasis, and TNM （Tumor, Node, Metastasis）staging of gastric carcinoma （P〈0.05）. Spearman rank correlation a

关 键 词：TIAM-1 HPA-1 肝癌 侵袭转移 实时荧光定量PCR 

分 类 号：R735.7[医药卫生—肿瘤]