液相芯片技术检测痰湿体质人群TNF-α、IL-6、CRP及MCP-1的表达研究  

Expressions of TNF-α, IL- 6, CRP, and MCP- 1 in Phlegm-damp Constitution Population Detected by Multiplexed Luminex Assay

作　　者：郑璐玉[1] 杨玲玲 李玲孺[1] 井慧如[1] 王济[1] 王前飞[2] 王琦[1] 

机构地区：[1]北京中医药大学基础医学院中医体质研究中心,北京100029 [2]中国科学院北京基因组研究所,北京100029

出　　处：《中国中西医结合杂志》2013年第7期920-923,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81030064);国家自然科学青年基金资助项目(No.81102526)

摘　　要：目的应用液相芯片技术研究20～50岁痰湿体质及平和体质人群外周血血清炎症因子——肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、C-反应蛋白(C-reactive protein,CRP)及单核细胞趋化蛋白-1(monocyte chemotactic protein1,MCP-1)的表达变化及其与肥胖的关系。方法选择2011年4—12月北京普仁医院体检中心体检人群101名。以体重指数(BMI)为依据,将研究对象分为痰湿体质肥胖组(OBT,BMI≥24kg/m2)30例,痰湿体质非肥胖组(NOBT,BMI<24kg/m2)25例,肥胖非痰湿体质组(OBNT,BMI≥24kg/m2)28例以及平和体质组(P,BMI<24kg/m2)18例。比较各组BMI及体脂肪率(FAT%);采用液相芯片法测定各组血清TNF-α、IL-6、CRP及MCP-1水平。结果 OBT和OBNT组BMI显著高于NOBT及P组,差异有统计学意义(P<0.05);OBT和OBNT组体FAT%高于P组,差异均有统计学意义(P<0.01)。OBT组血清TNF-α高于P组,差异有统计学意义(P<0.01);OBT、OBNT组及NOBT体质组血清CRP及MCP-1含量均高于P组,差异有统计学意义(P<0.05,P<0.01);痰湿体质评分与TNF-α、IL-6及MCP-1呈正相关(P<0.05)。结论 20～50岁痰湿体质人群存在炎症因子异常,且痰湿体质人群和肥胖人群均存在慢性低度炎症状态。Objective To study the expression changes of tumor necrosis factor-α （TNF-α）, interleukin- 6 （ IL- 6）, C-reactive protein （CRP）, monocyte chemotactic protein 1 （ MCP- 1 ）, and their cor- relation with obesity in 20 -50 years old population of phlegm-damp constitution （PDC） and of normal constitution （NC） using Luminex technique. Methods Totally 101 population were recruited from Health Examination Center of Puren Hospital from April to December 2011. Based on body mass index （BMI）, the subjects were assigned to four groups, i.e., the obesity of PDC group （Group OBT, BMI≥24 kg/m^2, 30 cases）, the non-obesity of PDC group （Group NOBT, BMI 〈24 kg/m^2, 25 cases）, the obesity of non- PDC group （Group OBNT, BMI≥24 kg/m^2, 28 cases）, the NC group （Group P, BMI 〈24 kg/m^2, 18 cases ）. The BMI and body fat percent （ FAT% ） were compared among the 4 groups. Serum levels of TNF-α, IL-6, CRP, and MCP-1 were measured with Luminex technique. Results BMI was significantly higher in Group OBT and Group OBNT than in Group NOBT and Group P （alIP 〈0.05）. The FAT% was significantly higher in Group OBT and Group OBNT than in Group P （P 〈0.01 ）. The serum TNF-α level in Group OBT was higher than in Group P （P 〈0.01 ）. The serum CRP and MCP- 1 levels were significantly higher in Group OBT, NOBT, and OBNT than in Group P （P 〈0.05,P 〈0.01 ）. The score for PDC was positively correlated with TNF-α, IL- 6, and MCP- 1 levels （P 〈 0.05）. Conclusions Abnormal higher levels of in- flammatory factors exist in 20 -50 years old population of PDC. Chronic inflammation exists in population of PDC and obesity people.

关 键 词：痰湿体质 炎症因子 液相芯片 

分 类 号：R229[医药卫生—中医基础理论]