白细胞介素6对人胰腺癌细胞上皮间质转换的影响及机制探讨  

The effects and mechanism of IL-6 on the epithelial to mesenchymal transition of pancreatic cancer cells

作　　者：杨光[1] 李雪飞[1] 崔刚[1] 裘正军[2] 黄陈[2] 

机构地区：[1]泰安市中心医院普外科,271000 [2]上海交通大学附属第一人民医院普外科

出　　处：《中华肝胆外科杂志》2013年第7期539-543,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金项目（81101844,81210108027）;上海市人才发展资金项目（2012040）

摘　　要：目的应用白细胞介素6（IL-6）作用于人胰腺癌细胞株SW1990、Capan-2和沉默STAT3基因的SW1990细胞，观察细胞上皮间质转换相关基因的变化并探讨其机制。方法使用IL-6作用人胰腺癌细胞株SW1990、Capan-2和沉默STAT3基因的SW1990细胞。MTT法检测细胞增殖。荧光定量PCR、RealtimePCR、Westernblot检测STAT3、PSTAT3、Snail、Twist和E—cadher—in基因mRNA和蛋白表达。体外侵袭实验检测细胞的侵袭能力。结果100μg／LIL-6作用人胰腺癌细胞株后，细胞增殖能力增强（P〈0．05）。Westernblot显示P—STAT3的表达增加；荧光定量PCR、Real—timePCR、Westernblot显示SnailmRNA和蛋白表达明显升高（P〈0．05）；E—cadherinmRNA和蛋白表达明显降低（P〈0．05）；细胞侵袭能力增强。而IL-6作用沉默sTAT3基因的SW1990细胞，Snail和EcadherinmRNA和蛋白表达均无明显改变。结论IL-6可能通过激活STAT3信号转导通路，上调Snail和下调E—eadherin基因表达，促进胰腺癌细胞上皮间质转换，进而增强侵袭能力。Objective To investigate the effects and mechanism of IL 6 on the epithelial to mes- enchymal transition of human pancreatic cancer cells. Methods IL-6 was added into the culture media of human pancreatic cancer cells Capan-2, SW1990, and STAT3-siRNA-SW1990. Cell growth was measured by MTT assays. STAT3, p STAT3, Snail, Twist, and E-cadherin mRNA and protein ex- pression were examined using real-time fluorescence quantitative polymerase chain reaction （RT-PCR） and Western blot, respectively. The invasion abilities of SW1990 and Capan-2 cells were determined by a cell invasion assay in vitro. Results Our results showed that 100 μg/L of IL-6 significantly pro- moted the growth and invasion abilities of Capan-2 and SW1990 cells （P〈0.05）. The use of IL 6 not only markedly increased the protein expression of P-STAT3 and Snail, but also greatly decreased the mRNA and protein expression of E cadherin. The use of IL 6 can not change the mRNA and protein expression of Snail and E cadherin. Conclusion Activation of the STAT3 signal transducer pathway with IL-6 can promote the epithelial to mesenchymal transition of pancreatic cancer cells in vitro through up regulation of Snail and down-regulation of E-cadherin expression. Therefore the STAT3 signal transducer may provide a novel therapeutic target for the treatment of pancreatic cancer.

关 键 词：胰腺肿瘤 白细胞介素6 信号传导 上皮 

分 类 号：R735.9[医药卫生—肿瘤]