诱导金属硫蛋白表达对大鼠缺血再灌注心肌细胞凋亡及核因子κB表达的影响  

Effects of induced metallothionein on myocardial apoptosis and expressions of nuclear factor-kappa B in rat hearts during ischemia/reperfusion

作　　者：庄梅[1] 雷大卫[1] 

机构地区：[1]贵阳医学院附属医院心脏内科,贵州贵阳550004

出　　处：《中国老年学杂志》2013年第13期3099-3101,共3页Chinese Journal of Gerontology

基　　金：贵州省科学技术基金〔黔科合J字(2007)2099〕

摘　　要：目的探讨金属硫蛋白(MT)对大鼠缺血再灌注(I/R)心肌细胞凋亡及核因子κB(NF-κB)表达水平的影响。方法 32只SD大鼠随机分成实验组、对照组,每组16只。分别予硫酸锌(ZnSO4)和蒸馏水预处理。预处理后构建Langendorff离体心脏I/R动物模型,动态观测缺血前及再灌注期左心室发展压(LVDP)、左心室压力最大上升和下降速率(±dp/dtmax)、冠状动脉流出量(CF);测定冠状动脉流出液肌酸磷酸激酶MB同工酶(CK-MB)的漏出率;Western印迹法检测金属硫蛋白(MT)表达;TUNEL法检测心肌细胞凋亡;免疫组化法检测NF-κB的活化水平。结果与对照组比较,实验组再灌注期LVDP、±dp/dtmax及CF得到改善(P<0.05);CK-MB的漏出率减少;金属硫蛋白的表达增加;细胞凋亡指数及NF-κB表达减少(均P<0.01)。结论 MT下调NF-κB表达可能与其抑制I/R心肌细胞凋亡有关。Objective To explore the effects of induced metallothionein（MT） on myocardial apoptotic and nuclear factor-kappa B（NF-κB） expression in rat hearts during ischemia/reperfusion.Methods 32 SD rats were divided randomly into the experiment and control groups.Rats had been pretreated with 3.6% ZnSO4 or distilled water before their hearts were separated for Langendorff perfusion and for ischemia/reperfusion.The left ventricular functions（LVDP,±dp/dtmax）,coronary artery flows（CF） were observed dynamically before ischemia and during 60-minute reperfusion following 30-minute ischemia.The levels of CK-MB in the coronary artery flow were measured.Apoptosis was measured by TUNEL staining method.MT was assessed by using western blot.NF-κB activation was examined by using immunohistochemistry.Results Compared with control group,the LVDP,±dp/dtmax and CF were greatly improved（P0.05）,the level of CK-MB,the apoptotic index and the activation of NF-κB were significantly decreased（P0.01） in experiment group.Conclusions MT can induce down-regulation of NF-κB,which function might inhibit myocardial apoptosis after ischemia/reperfusion in rat.

关 键 词：缺血再灌注 金属硫蛋白 细胞凋亡 核因子ΚB 

分 类 号：R363[医药卫生—病理学]