大黄素对肝纤维化大鼠肝脏组织中Ⅰ型前胶原mRNA表达的影响  

Effects of emodin on expression of procollagen α1(Ⅰ) mRNA in liver of rats with hepatic fibrosis induced by carbon tetrachloride

作　　者：董妙先[1] 綦艳秋[1] 朱兰芹[1] 耿玉涛[2] 李佳鸣[2] 邹德嘉[2] 王刚[2] 

机构地区：[1]齐齐哈尔医学院卫生所,齐齐哈尔市161006 [2]齐齐哈尔医学院医药科学研究所

出　　处：《齐齐哈尔医学院学报》2013年第14期2029-2030,共2页Journal of Qiqihar Medical University

基　　金：黑龙江省教育厅科学技术研究面上项目(No.11551551)

摘　　要：目的观察大黄素对四氯化碳(CCl4)诱导肝纤维化大鼠肝脏组织Ⅰ型前胶原mRNA表达的影响。方法 50只雄性SD大鼠随机分为空白对照组(n=10)、CCl4组(n=20)和大黄素+CCl4组(n=20),共3组。采用40%CCl4橄榄油混悬液2 ml/kg皮下注射(每周2次,共12周)法制备大鼠肝纤维化模型,同时大黄素灌胃干预,12周后处死大鼠。采用实时定量PCR法检测肝脏组织Ⅰ型前胶原mRNA表达。结果与空白对照组比较,CCl4组大鼠肝脏组织Ⅰ型前胶原mRNA表达显著增加;与CCl4组比较,大黄素可显著减少肝脏组织Ⅰ型前胶原mRNA表达。结论大黄素具有下调纤维化肝脏组织Ⅰ型前胶原mRNA表达的作用。Objective To observe the effect of emodin on procollagen α1（I） mRNA expression in rat liver in hepatic fibrogenesis induced by carbon tetrachloride（CCl4）.Methods Fifty male SD rats were randomly divided into 3 groups,named as blank control group（n = 10）,CCl4 group（n = 20）,and emodin ＋ CCl4 group（n = 10）.The rat models of experimental hepatic fibrosis were established by injection with CCl4.The treated rats received emodin via oral administration at a dosage of 20mg / kg / d at the same time.The mRNA expressions of procollagen α1（I） were assessed using real-time RT-PCR.Results The mRNA expression of procollagen α1（I） in the CCl4 group was significantly up-regulated than that in the blank control group,and that from CCl4 ＋ emodin group was significantly down-regulated than that in the CCl4 group（P 0.05）.Conclusions Emodin can give a down-regulate expression of procollagen α1（I） mRNA in fibrotic liver tissue.

关 键 词：肝纤维化 Ⅰ型前胶原 实时定量PCR 大鼠 

分 类 号：R734.2[医药卫生—肿瘤]