HFJ大鼠与Wistar大鼠用于动脉粥样硬化造模的比较研究  

Comparative Study of HFJ Rats with Wistar Rats on the Animal Models for Atherosclerosis Disease

作　　者：连伟光[1] 郑龙[1] 尤红煜[1] 刘健敏[1] 张东明[1] 刘福英[1] 王俊霞[1] 

机构地区：[1]河北医科大学,河北省实验动物重点实验室,石家庄050017

出　　处：《四川动物》2013年第4期588-594,共7页Sichuan Journal of Zoology

基　　金：河北省卫生厅青年课题资助(20110040);河北省科技条件建设项目(11965518D)

摘　　要：目的通过高脂喂养和免疫损伤结合的方法,建立HFJ近交系大鼠和Wistar封闭群大鼠动脉粥样硬化动物模型并进行比较分析。方法选择HFJ近交系和Wistar封闭群大鼠,分别随机分为模型组和正常组,正常组给予基础饲料饲喂,模型组给予高脂饲料饲喂,并采用牛血清白蛋白(40mg/kg)和卵清白蛋白(2.5mg/kg)进行免疫损伤,并辅以维生素D3(25万U/kg)灌胃,饲养90d后测定血脂水平、血液生化指标、观察病理变化和血管内皮生长因子(VEGF)免疫组化情况。结果 (1)HFJ和Wistar大鼠正常组相比较,前者TG、TC和LDL-C水平高于后者(P<0.05),HFJ大鼠模型组LDL-C含量明显高于Wistar大鼠(P<0.05);(2)心肌损伤指标,HFJ和Wistar大鼠模型组均较正常组心肌激酶(CK)、心肌激酶同工酶(CK-Mb)明显升高;(3)HE染色发现与Wistar大鼠模型组相比,HFJ近交系大鼠斑块形成更为明显,明显处于动脉粥样硬化病理Ⅲ期,可见纤维帽形成,纤维帽下具有典型的胆固醇结晶裂隙和泡沫细胞,中层平滑肌排列紊乱;(4)免疫组化法测定主动脉弓VEGF蛋白的表达,HFJ大鼠模型组较Wistar大鼠表达升高(P<0.05)。结论成功建立了HFJ大鼠动脉粥样硬化疾病动物模型,与Wistar大鼠相比HFJ大鼠模型特点更为显著,可为动脉粥样硬化研究提供一新的实验动物品系。Objective To establish and compare atherosclerosis models of Wistar closed colony rats and high fecundity inbred HFJ rats by feeding high-fat diets and immune injury. Methods Male Wistar rats and HFJ inbred rats were random- ly divided into normal group and model group. The normal group was fed with basic diet. The model group was fed a high- fat diet and received immune injury （bovine serum albumin 40 m^kg, chicken egg albumin 2.5 mg/kg） and gastric perfu- sion of vitamin D3. The serum lipid level, biochemical indices and pathological changes were examined after 90 days. The expression of vascular endothelial growth factor （VEGF） in the aortic arch was detected by immunehistochemical tech- niques. Results （ 1 ） In normal group, the values of TG, TC and LDL-C in t-IFJ inbred rats were significantly higher than in Wistar rats （ P 〈 0.05 ）. The production of LDL-C in model group of HFJ rats also showed a higher level than in Wistar rats （ P 〈 0.05 ） ; （2） The content of CK and CK-Mb in model groups were statistically higher than those of normal animals （ P 〈 O. 05 ） ; （ 3 ） After HE staining, typical pathologic stage ]]I characters of atheromatous plaques was observed in the model group of HFJ inbred rats rather than in Wistar rats ; （4） The expression of VEGF in the model group HFJ inbred rats was higher than in Wistar rats as determined by immunehistochemical techniques （P 〈 O. 05）. Conclusion An atheroscle- rosis mouse model was successfully developed based on HFJ inbred rats, which could provided a new strain of laboratory ani- mal for AS researches.

关 键 词：动脉粥样硬化 动物模型 免疫损伤 HFJ大鼠 

分 类 号：R-332[医药卫生]