Zn^(2+)对基因工程大肠杆菌P84A/MC1061发酵生产卤醇脱卤酶的影响  

Influence of Zn^(2+) on the Halohydrin Dehalogenase Produced by Recombinant Escherichia coli P84A/ MC1061

作　　者：潘冬瑞[1] 李啸[1,2] 张瑶[1] 李知洪[2] 俞学锋[2] 

机构地区：[1]三峡大学化学与生命科学学院,湖北宜昌443003 [2]安琪酵母股份有限公司,湖北宜昌443003

出　　处：《天津农业科学》2013年第8期5-9,共5页Tianjin Agricultural Sciences

摘　　要：主要研究Zn2+对基因工程大肠杆菌发酵生产卤醇脱卤酶的影响。以卤醇脱卤酶活力为响应值,通过单因素试验筛选出显著影响因子Zn2+,并确定其最优浓度为0.0007g·L-1;在50L罐中进行分批发酵试验,比较分析卤醇脱卤酶活力、OD600以及在线参数OUR、CER等的变化趋势,发现Zn2+可显著促进重组大肠杆菌生长,提高生长速率,酶活提高了5.4%。Studied the influence of Zn2＋ on the halohydrin dehalogenase produced by recombinant Escherichia coli P84A/MC1061. First,to regard halohydrin dehalogenation enzyme activity as the response value, determine the significant factor was Zn2＋ and the optimal concentration was 0.000 7 g·L -1 by single factor experiments;then,it showed that Zn2＋ could significantly promote the growth of recombinant E.Coli and increase the growth rate, and the enzyme activity was a 5.4% increase compared with the control group by comparing and analyzing OD 600 , halohydrin dehalogenase enzyme activity and online parameters OUR,CER and RQ in 50 L batch fermentation experiments.

关 键 词：卤醇脱卤酶 锌离子 大肠杆菌 发酵调控 

分 类 号：R378.21[医药卫生—病原生物学]