白灵侧耳结实相关基因片段的筛选与分析  

Screening and Analysis of Gene Fragments Related to Fructification of Pleurotus eryngii var. tuoliensis

作　　者：陈苗苗[1,2] 黄晨阳[2] 陈强[2] 赵梦然[2] 李明[1] 张金霞[2] 

机构地区：[1]河北农业大学园艺学院,河北保定071000 [2]农业部农业微生物资源收集与保藏重点实验室/中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《生物技术》2013年第3期4-8,共5页Biotechnology

基　　金：现代农业产业技术体系建设专项资金项目(编号:CARS24)资助~~

摘　　要：目的:寻找白灵侧耳结实相关基因,探寻白灵侧耳结实机理。方法:通过mRNA差异显示技术筛选白灵侧耳菌丝和原基两个发育阶段差异表达的基因片段,经半定量PCR验证后,应用生物信息学方法对片段进行同源性分析并预测其基因功能。结果:共获得8条差异片段,1条与未知蛋白具有40%同源性的基因片段在菌丝中高表达,其余7条在原基中高表达,与细胞色素P450、糖基转移酶、扩展蛋白、糖苷水解酶家族3、核糖体蛋白L29、高半胱氨酸甲基转移酶、未知蛋白的氨基酸同源性为32%～88%不等。结论:差异基因的获得为食用菌结实机理的研究及育种奠定基础。Objective： To obtain the fructification related genes and understand the fructification mechanism of Pleurotus eryngii var. tuoliensis. Method：The differences of gene expression between the two developmental stages of primordia and mycelia were analyzed by mRNA differential display. The authenticity of different expression was identified by semi - quantitative RT - PCR. Homology analysis of the target gene was carried out by bioinformatics. Result： Eight differential cDNAs were selected. One of them was highly expressed in my- celia,which shared 40% homology with a hypothetical protein. The other cDNAs were highly expressed in primordia,which shared 32% - 88% homology with the genes coding for Cytochrome P450, Glycosyltransferase -related protein, Non -Catalytic module family EXPN pro- tein, Glycoside hydrolase family 3, ribosomal protein L29, Homocysteine S - methyltransferase and hypothetical protein, respectively. Conclusion：This study plays a fundamental role in understanding the mechanism of fructification and breeding in edible mushroom.

关 键 词：菌丝 原基 结实 MRNA差异显示技术 

分 类 号：S646[农业科学—蔬菜学]