PRP/nHA/Co复合材料促进牵张成骨的实验研究  

Experimental study of PRP/nHA/Co compound in distraction osteogenesis

作　　者：张莉[1] 边晓为[2] 姚望[1] 马宁[3] 

机构地区：[1]吉林大学口腔医院急诊科,长春130021 [2]吉林大学口腔医院科教部,长春130021 [3]吉林大学口腔医院牙周病科,长春130021

出　　处：《实用口腔医学杂志》2013年第4期477-481,共5页Journal of Practical Stomatology

基　　金：吉林省科技厅社会发展处课题重点项目(编号:20090437);吉林省科技厅基础处课题项目(编号:201115107)

摘　　要：目的:探讨富含血小板血浆(PRP)、纳米羟基磷灰石(nHA)和胶原(Co)复合材料PRP/nHA/Co促进牵张成骨的效果。方法:取18只健康成年的新西兰大耳兔,建立双侧下颌骨牵张成骨模型。随机分为3组(n=6);A组骨切开处植入PRP/nHA/Co复合膜材料,B组植入nHA/Co复合膜,C组为空白对照组,不放置任何材料。术后4周、8周分别处死动物,取双侧下颌骨,行X线照相,常规切片HE染色,BMP-2及bFGF免疫组织化学染色,光镜下观察。结果:X线、HE组织学观察PRP/nHA/Co组新骨形成均优于其他2组,免疫组织化学观察牵张4周时BMP-2和bFGF表达高于其他2组,8周时表达减弱。结论:PRP/nHA/Co复合材料能够促进牵张成骨。Objective：To evaluate the effects of PRP/nHA/Co compound in distraction osteogenesis.Methods： Bilateral mandibular distraction osteogenesis model was established in 18 healthy adult New Zealand big ear rabbits.Compound membrane materials of collagen I（Co）,nanohydroxyapatie（nHA） and platelet rich plasma（PRP） were prepared as PRP/nHA/Co and nHA/Co,and implanted into the corresponding position of rabbits in group A and group B respectively,in group C no implant was used.After 4 and 8 weeks rabbits were killed respectively,bilateral mandibula were examined by X ray photography,HE staining,BMP-2 and bFGF immunohistochemical staining.Results： New bone formation in PRP/nHA/Co group was superiorer than in other 2 groups.At 4th week of distraction BMP-2 and bFGF expressions in distraction area in PRP/nHA/ Co group were stronger than in other 2 groups,and decreased at the 8th week.Conclusion： PRP/nHA/Co compound material may promote distraction osteogenesis.

关 键 词：组织工程 牵张成骨 羟基磷灰石 胶原 富含血小板血浆 

分 类 号：R783[医药卫生—口腔医学]