氯化镧对体外培养的人牙周膜细胞生物学活性的影响  

Influences of lanthanum chloride on the bioactivities of human periodontal ligament cells cultured in vitro

作　　者：许萍[1] 王丽琴 荆得宝 王小平[1] 

机构地区：[1]同济大学口腔医院,上海200072 [2]上海市口腔病防治院 [3]上海市浦东新区公立医院口腔科

出　　处：《实用口腔医学杂志》2013年第4期516-520,共5页Journal of Practical Stomatology

基　　金：上海市浦东新区社会发展局卫生科技项目(编号:PW2009A-6);上海市科委重点攻关项目(编号:074119614)

摘　　要：目的:探讨稀土化合物氯化镧对人牙周膜细胞(HPDLCs)生物学活性的影响。方法:选择10～2mol/L～10-10mol/L的氯化镧作用于人牙周膜细胞,MTT法评价细胞增殖率,HE及DAPI染色观察细胞及核的形态学特征,单细胞凝胶电泳法检测DNA损伤,流式细胞仪检测细胞周期的变化。结果:10-2mol/L和10-3mol/L组氯化镧对牙周膜细胞的增殖有明显的抑制作用,并使细胞的原有形态发生了改变;10-4mol/L组仅在细胞周期检测中可见到显著性差异;10-5mol/L以下浓度对牙周膜细胞的增殖无明显影响,10-10mol/L组显示出促增殖作用。结论:高浓度的氯化镧对牙周膜细胞有毒性损伤作用,10-5mol/L以下浓度对牙周膜细胞的生物学活性未见不良影响,极低浓度组可促进牙周膜细胞增殖。Objective：To study the influences of lanthanum chloride on the the bioactivities of human periodontal 1igament cells（HPDLCs） in vitro.Methods： HPDLCs were treated by lanthanum chloride at 10^-2 mol/L to 10^-10 mol/L.Cell proliferation was examined by MTT assay,cell morphology was observed by hematoxylin-eosin staining and DAPI staining,DNA damage was detected by single cell gel electrophoresis（SCGE） assay and cell cycle distribution was analysed by flow-cytometry.Results： Lanthanum chloride at 10^-2 mol/L and 10^-3 mol/L promored the proliferation,induced DNA damages and cell cycle changes of HPDLCs,at 10^-10 mol/L increased cell proliferation.Conclusion： High concentration of lanthanum chloride is toxic to HPDLCs,the concentration lower than 10^-5 mol/L has no effect on the bioactivities,very low concentration may promote the cell proliferation.

关 键 词：氯化镧 牙周膜细胞 细胞增殖 凋亡 毒性 

分 类 号：R780.2[医药卫生—口腔医学]