鼻咽癌患者治疗过程中血清EB病毒抗体水平动态变化与近期疗效的关系  

Correlation between short-term response and the dynamic changes of serum levels of anti-Epstein-Barr virus antibodies in patients with nasopharyngeal cancer when ungoing radiation therapy

作　　者：李军 汤敏中[1,2] 陆爱英 钟伟铭 高健全[3] 郑裕明 曾洪 蔡永林 

机构地区：[1]梧州市卫生系统鼻咽癌病因学及分子机理重点实验室,广西壮族自治区梧州543002 [2]北京工业大学生命科学与生物工程学院,北京100022 [3]梧州市红十字会医院肿瘤科,广西壮族自治区梧州543002

出　　处：《肿瘤》2013年第7期624-628,633,共6页Tumor

基　　金：广西科学研究与技术开发计划项目(编号:11194011);广西本级技术研究与开发项目(编号:114003A-49);梧州市科学研究与技术开发计划项目(编号:200901066)

摘　　要：目的:检测鼻咽癌患者放射治疗期间血清Epstein-Barr(EB)病毒VCA/IgA、EA/IgA、NA1/IgA及Rta/IgG抗体水平的变化,探讨其与鼻咽癌近期疗效的关系。方法:采集140例初治时无远处转移的鼻咽癌患者在放射治疗前、治疗中(放疗剂量达40Gy时)以及治疗结束后的血清标本,应用间接免疫酶法检测VCA/IgA和EA/IgA水平,ELISA法检测NA1/IgA和Rta/IgG水平。治疗结束后3个月观察鼻咽癌患者的近期疗效,并分析其血清EB病毒抗体水平与近期疗效的相关性。结果:放射治疗后鼻咽原发灶和颈部淋巴结转移灶达完全缓解以及部分缓解的患者在治疗过程中其血清VCA/IgA、EA/IgA及Rta/IgG抗体水平均呈显著下降趋势(P<0.05),NA1/IgA抗体水平则无明显变化(P>0.05),但完全缓解患者和部分缓解患者之间EB病毒抗体水平差异并无统计学意义(P>0.05)。治疗后随访1年内发生远处转移的患者其治疗前EB病毒抗体水平与未发生远处转移的患者比较亦未见明显差异(P>0.05)。结论:血清EB病毒VCA/IgA、EA/IgA、NA1/IgA及Rta/IgG抗体水平可能与鼻咽癌经放射治疗后的近期疗效无关。Objective： To investigate the changes of serum levels of antibodies against EBV （Epstein-Barr virus） including VCA/IgA, EA/IgA, NA1/IgA and Rta/IgG in patients with NPC （nasopharyngeal cancer） during radiotherapy, and to explore the correlation between the serum levels of anti-EBV antibodies and the short-term response to radiation therapy. Methods： Serum samples from 140 primary NPC patients without distant metastasis were collected before radiation therapy and when the radiation dose reached 40 Gy as well as the radiation therapy was finished. The titers of VCA/IgA and EA/IgA were detected using an indirect immunoenzyme assay, and the titers of NA1/IgA and Rta/IgG were detected using ELISA （enzyme-linked immunosorbent assay）. At the third month after radiation therapy finished, the short-term response in NPC paitents was observed. The correlation of short-term response and the serum levels of anti-EBV antibodies was analyzed. Results： The serum levels of VCA/IgA, EA/IgA and Rta/IgG were significantly decreased during the period of radiation therapy in patients achieving CR （complete remission） or PR （partial remission） in primary lesion or cervical lymph nodes （P 〈 0.05）, but the serum level of NA1/IgA had no significant change （P 〉 0.05）. No statistical differences in serum levels of anti-EBV antibodies were found between patients achieving CR and PR （P 〉 0.05）. There was also no significant difference in the pre-treatment serum levels of anti-EBV antibodies between patients with distant metastasis and those without distant metastasis during 1-year follow-up after treatment （P 〉 0.05）. Conclusion： The serum levels of antibodies against EBV including VCA/IgA, EA/IgA, NA1/IgA and Rta/IgG may not associated with the short-term response to radiation therapy in NPC patients.

关 键 词：鼻咽肿瘤 疱疹病毒4型 人 抗体 病毒 治疗结果 肿瘤消退 自行性 

分 类 号：R735.35[医药卫生—肿瘤]