Dexamethasone Reduces IL-17 and Tim-3 Expression in BALF of Asthmatic Mice  

作　　者：陆小霞 Karen S.McCoy 胡维琨 徐佳莉 王海勤 陈鹏 陈和斌 

机构地区：[1]Department of Pulmonary Medicine,Wuhan Women and Children Health Care Center [2]Department of Pediatrics,Division of Pulmonary Medicine,Nationwide Children's Hospital and The Ohio State University School of Medicine and Public Health [3]Department of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2013年第4期479-484,共6页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.81200006);the Natural Science Foundation of Hubei Province(No.2010CDB08804);Wuhan Health Bureau Foundation(No.WX11B10);supported by Wu Jieping Medical Fund(No.32067501285)

摘　　要：Summary： This study investigated the expression of interleukin-17 （IL-17） and T cell immunoglobulin mucin and domain-containing molecule-3 （Tim-3） in bronchoalveolar lavage fluid （BALF） of asthmatic mice and the effect of dexamethasone （DEX） on these factors. Thirty-six mice were randomly divided into three groups： normal group, asthmatic group and DEX group. The mouse model of asthma was es- tablished by sensitization with ovalbumin in both the asthmatic and DEX groups. The levels of IL-6, IL-10, IL-17 and TGF-β were measured in BALF by enzyme-linked immunesorbent assay （ELISA）. The mRNA expression level of Tim-3 was detected by reverse transcription polymerase chain reaction （RT-PCR）. The ratio of Tim-3＋CD4＋ cells to total CD4＋ cells in BALF was determined by flow cy- tometry. Differential inflammatory cells in BALF were detected. The correlations among IL-17, IL-6, IL-10, Tim-3 and inflammatory cells were analyzed. The results showed that the levels of IL-17, IL-6 and Tim-3 were substantially increased and the IL-10 level decreased in BALF in the asthmatic mice, which was significantly reversed by DEX treatment. IL-17 expression was positively correlated with IL-6 and Tim-3 expression and the number of inflammatory cells but negatively with IL-10 expression. These results indicate that the increased expression of IL-17 and Tim-3 in BALF may be implicated in the occurrence and development of asthmatic inflammation; the mechanism by which DEX suppresses asthmatic airway inflammation involves down-regulation of IL-17 and Tim-3 levels.Summary： This study investigated the expression of interleukin-17 （IL-17） and T cell immunoglobulin mucin and domain-containing molecule-3 （Tim-3） in bronchoalveolar lavage fluid （BALF） of asthmatic mice and the effect of dexamethasone （DEX） on these factors. Thirty-six mice were randomly divided into three groups： normal group, asthmatic group and DEX group. The mouse model of asthma was es- tablished by sensitization with ovalbumin in both the asthmatic and DEX groups. The levels of IL-6, IL-10, IL-17 and TGF-β were measured in BALF by enzyme-linked immunesorbent assay （ELISA）. The mRNA expression level of Tim-3 was detected by reverse transcription polymerase chain reaction （RT-PCR）. The ratio of Tim-3＋CD4＋ cells to total CD4＋ cells in BALF was determined by flow cy- tometry. Differential inflammatory cells in BALF were detected. The correlations among IL-17, IL-6, IL-10, Tim-3 and inflammatory cells were analyzed. The results showed that the levels of IL-17, IL-6 and Tim-3 were substantially increased and the IL-10 level decreased in BALF in the asthmatic mice, which was significantly reversed by DEX treatment. IL-17 expression was positively correlated with IL-6 and Tim-3 expression and the number of inflammatory cells but negatively with IL-10 expression. These results indicate that the increased expression of IL-17 and Tim-3 in BALF may be implicated in the occurrence and development of asthmatic inflammation; the mechanism by which DEX suppresses asthmatic airway inflammation involves down-regulation of IL-17 and Tim-3 levels.

关 键 词：ASTHMA Thl7 cells TIM-3 CYTOKINES CORTICOSTEROIDS 

分 类 号：R562.25[医药卫生—呼吸系统]