早期母爱剥夺促发抑郁并增加癫痫易感性  

Early maternal deprivation induces depression and increases the susceptibility to epilepsy in rats

作　　者：潘楠楠[1] 王敏[2] 冯海燕[2] 郑乃智[1] 

机构地区：[1]青岛大学,在读研究生青岛266011 [2]青岛市市立医院神经内科

出　　处：《中国神经精神疾病杂志》2013年第7期406-410,共5页Chinese Journal of Nervous and Mental Diseases

摘　　要：目的观察母爱剥夺应激及癫痫发作后大鼠海马颗粒层细胞数第1周及4周的变化,了解母爱剥夺应激对大鼠行为学的影响,进一步揭示其对癫痫发作的影响。方法新生SD雄性大鼠随机分为母爱剥夺组与对照组,大鼠出生当天(postnatal day,P)记为P0。母爱剥夺组大鼠P1至P14,每天给予母爱剥夺应激,对照组不予任何处理;P21两组大鼠断奶,监测体重并记录每日体重增长量;P27进行糖水偏爱实验。随后再将上述两组大鼠随机分为两组,癫痫组接受匹鲁卡品建立癫痫模型,生理盐水组接受同等剂量生理盐水。采用NeuN免疫荧光染色计数大鼠海马颗粒层细胞数。结果母爱剥夺组大鼠体重(55.33±5.17 g)及每日体重增长量(2.78±0.36 g)显著低于对照组(78.64±1.51 g、5.84±1.03 g,P<0.05)。母爱剥夺组大鼠蔗糖偏爱比例(59.29%±5.59%)与对照组相比(77.06%±3.34%)显著降低(P<0.05),母爱剥夺组大鼠蔗糖消耗比例(66.17±4.58 mL/Kg)较对照组(85.90±7.54 mL/Kg)显著减少。给予匹鲁卡品后,母爱剥夺组大鼠首次出现癫痫发作的时间(3.62±0.37 min)较对照组显著缩短(6.37±0.59 min,P<0.05)。母爱剥夺组大鼠首次达到Racine’s法Ⅳ级发作的时间(8.87±0.87min)较对照组显著缩短(13.50±1.16 min,P<0.05)。NeuN免疫荧光染色示母爱剥夺组大鼠第1(31.80±0.91个/视野)及第4周(37.50±0.73个/视野)海马颗粒层细胞数较对照组(39.00±0.77个/视野)显著减少(P<0.05),而癫痫组大鼠第1(41.30±0.80个/视野)及第4周(43.60±0.85个/视野)海马颗粒层细胞数较对照组(41.00±0.63个/视野)显著增加(P<0.05)。结论母爱剥夺应激可以导致抑郁样行为学改变,并增加癫痫易感性。Objective To assess whether early maternal deprivation can induce the alteration of the hippocampal granular layer and explore the effects of early maternal deprivation on depression and susceptibility to epilepsy. Methods Newborn SD male rats were randomly divided into maternal deprivation group （n=20） and control group （n=20）. Rats in ma-ternal deprivation group experienced maternal deprivation for 3 h （9：00-12：00 am） every day from PI to PI4 while rats in control group received no treatment. At P21, all rats were weaned and their body weights were recorded every day. From P27-P34 all rats received sucrose consumption test. Animals were then randomly divided into two subgroups. One sub- group of rats received pilocarpine （340 mg/Kg iv） to induce seizures while the other subgroup received saline only. The first onset of seizures and the time to first onset of Racine＇ s Ⅳ degree seizures were recorded. Rats were deeply anesthe-tized and perfused at 1 （control±pilocarpine： n= 5; maternal deprivation ±saline： n=5;control±saline：n=5） and 4 weeks （control±pilocarpine：n=5; maternal deprivation±saline： n=5;control±saline：n=5） after pilocarpine injection. Immunofluores-cent staining was used to examine the number of neurons of the granular layer. Results Compared with control group （78.64 ± 1.51 g, 5.84±1.03 g）, maternal deprivation group had significantly lower bodyweight （55.33 ± 5.17 g, P 〈 0.05）, lower growth of body weight （2.78±0.36 g, P 〈 0.05）, and lower sucrose preference rate （P 〈 0.05）. After pilocarpine injec-tion, the time of first onset of seizures （3.62±0.37 min） and the time to first onset of Racine＇s IV degree seizures （8.87±0.87 min）were significantly shorter in maternal deprivation group. Compared with control group, the number of NeuN posi-tive neurons in granular layer （31.80±0.91 cells/field 37.50±0.73 cells/field） were significantly decreased in maternal de-privation group while the number of NeuN positiv

关 键 词：母爱剥夺 抑郁 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]