体外培养大鼠小脑神经元划痕模型的建立  

Establishment of a scratch assay model using cerebellar neurons of rats in vitro

作　　者：张庆慧[1] 罗红凤[2] 赵炜疆[1] 

机构地区：[1]汕头大学医学院神经科学中心,汕头515041 [2]汕头大学医学院第一附属医院儿科,汕头515041

出　　处：《神经解剖学杂志》2013年第4期395-398,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81171138)

摘　　要：目的:建立体外培养SD大鼠小脑神经元划痕模型。方法:用200μl塑料枪头划痕体外培养大鼠小脑神经元,观察相同视野下不同时间点(0,24,48 h)的划痕宽度。结果:倒置相差显微镜下可见划痕细胞后0 h即形成划痕区,边缘整齐。24 h后,划痕边缘变模糊同时划痕宽度变窄。48 h后,划痕宽度进一步缩小。结论:本模型简便经济,能较好地模拟脑损伤后神经元的迁移。Objective ： To establish a scratch assay model by using the cerebellar neurons of SD rats in vitro. Methods： Scratch was induced by a 200μl plastic pipette tip on cerebellar neurons of rats in vitro. The widths of scratch at different time points （0 h, 24 h, 48 h） from the same field were measured. Results： An artificial scratch was created and the edge of the scratch was smooth at 0 h after scratching cell under an inverted phase microscope. At 24 h, the edge of the scratch blurred and the width became narrower. At 48 h, further reduction of the width of the scratch was observed. Con- clusion： This model proved to be a convenient and cost-effective method that can well mimic the cerebellar neurons migra- tion after brain injury.

关 键 词：小脑神经元 原代培养 划痕实验 细胞迁移 

分 类 号：R-332[医药卫生]