大鼠海马内注射β淀粉样蛋白1-40抑制铜蓝蛋白表达  

Decreasing ceruloplasmin expression induced by amyloid β-peptide(1-40) injection in rat hippocampus

作　　者：李艳伟[1] 赵晋英[1] 周泽江[1] 黄泽智[1] 李琳[2] 

机构地区：[1]邵阳医学高等专科学校分子生物学诊断重点实验室,邵阳422001 [2]山西医科大学教育部省部共建细胞生理学实验室,太原030001

出　　处：《神经解剖学杂志》2013年第4期421-426,共6页Chinese Journal of Neuroanatomy

基　　金：湖南省教育厅优秀青年项目(09B095);2010年湖南省大学生研究性学习和创新性实验计划项目(525)

摘　　要：目的:通过检测阿尔茨海默病(Alzheimer disease,AD)模型大鼠海马铜蓝蛋白(glycan-phosphatidyli-nositol ceruloplasmin,GPI-Cp)的变化,探讨AD时脑铁增高的机制。方法:取雄性(290±10)g SD大鼠,经海马内注射(amyloid beta-peptide 1-40,Aβ1-40)建立AD模型,于1、2、3和4周取各组大鼠脑组织,分离海马,用RT-PCR检测GPI-Cp mRNA表达情况,免疫组织化学染色检测GPI-Cp蛋白表达情况。结果:海马的星形胶质细胞、血管内皮细胞、室管膜细胞均有GPI-Cp的mRNA和蛋白表达;而海马的锥体细胞仅轻度表达,颗粒细胞不表达。注射Aβ1-40后,模型组海马GPI-Cp mRNA和蛋白表达均随着时间延长逐渐降低,具有统计学意义(P<0.01)。结论:海马内注射Aβ1-40可引起大鼠海马GPI-Cp表达减少,GPI-Cp表达减少可能是AD时脑铁增高的原因。Objective： To explore the effects of amyloid beta-peptide 1-40 （Aβ1-40） on ceruloplasmin （Cp） expres- sion in the hippocampus of rats. Methods： Male SD rats （290 ±10 g） were injected Aβ1-40 in the hippocampus and their hippocampuses were obtained after injection 1 week, 2 weeks, 3 weeks and 4 weeks, respectively. The expression of GPI-Cp mRNA was measured by reverse transcription polymerase chain reaction （RT-PCR）. The expression of GPI-Cp protein was examined by immunohistochemistry. Results：The expression of GPI-Cp mRNA and protein was shown in the hippocampus of rats, including epithelial cell of choroid plexus, ependymal cell, astrocyte of hippocampus and vascular endothelial cell, but mild in pyramidal cell and not in granulosa cell. The time-dependent decrease of GPI-Cp mRNA and protein expression were shown in the hippocampus of rats injected Aβ1-40 （P 〈 0.01 ）. Conclusion：In the hippocampus of rats, the Aβ1-40 injection induced the decrease of GPI-Cp expression,which may result in increase of iron concentra- tion of hippocampus and may be involved in the pathogenesis of AD.

关 键 词：阿尔茨海默病β淀粉样蛋白 脑铁含量 铜蓝蛋白 

分 类 号：R749.16[医药卫生—神经病学与精神病学]