PTPN22在溃疡性结肠炎中的表达及其临床意义  

Expression and Clinical Significance of PTPN22 in Ulcerative Colitis

作　　者：陈志涛[1] 夏冰[2] 张姮[1] 吴杰[1] 王萍[1] 姜挺[1] 宋敏[1] 

机构地区：[1]武汉市中心医院消化内科,430014 [2]武汉大学中南医院消化内科

出　　处：《胃肠病学》2013年第7期421-424,共4页Chinese Journal of Gastroenterology

基　　金：武汉市卫生局项目(WX12C34)资助

摘　　要：背景:溃疡性结肠炎(UC)的发生、发展与T细胞过度活化有关,蛋白酪氨酸磷酸酶非受体型22(PTPN22)参与T细胞活化的负性调节。目的:探讨PTPN22在UC中的表达及其临床意义。方法:纳入2010年7月～2012年7月武汉市中心医院、武汉大学中南医院就诊的UC患者60例,同时纳入35例IBS患者作为对照组。采用实时定量PCR法检测患者肠黏膜PTPN22 mRNA表达水平。采用全自动红细胞沉降系统分析仪检测血清ESR水平。采用速率散射比浊法检测血清CRP水平。结果:活动期UC患者肠黏膜PTPN22 mRNA表达水平与缓解期UC患者和对照组相比显著升高(P=0.007;P=0.021)。UC患者肠黏膜PTPN22 mRNA表达水平与血清ESR和CRP水平呈正相关(r=0.63,P=0.005;r=0.58,P<0.01)。UC患者疾病严重程度和病变范围与肠黏膜PTPN22 mRNA表达水平呈正相关(r=0.51,P<0.01;r=0.44,P<0.01)。中重度UC患者肠黏膜PTPN22 mRNA表达水平与轻度UC患者相比显著升高(P=0.005),病变位于广泛结肠者肠黏膜PTPN22 mRNA表达水平与病变位于左半结肠和直肠者相比显著升高(P=0.029;P=0.008)。结论:PTPN22 mRNA表达水平与UC疾病活动性、严重程度和病变范围相关。PTPN22可能在UC发病机制中发挥重要作用。Background： Excessive activation of T cell has been found to be related to the genesis and development of ulcerative colitis （UC）. Protein tyrosine phosphatase nonreceptor type 22 （PTPN22） plays a regulation role in T cell activation. Aims： To investigate the expression and clinical significance of PTPN22 in UC. Methods. A total of 60 UC patients at Wuhan Central Hospital and Zhongnan Hospital of Wuhan University from July 2010 to July 2012 were enrolled. Other 35 IBS patients were enrolled as control group. The expression of VI＇PN22 mRNA in colonic mucosa was determined by real- time quantitative PCR. The serum levels of ESR and CRP were measured by automatic ESR Analyzer SRS 100/I1 and rate nephelometry, respectively. Results： The expression of PTPN22 mRNA in UC patients in active stage was significantly higher than that in UC patients in remission stage and control group （P = 0.007 ; P = 0. 021 ）. The expression of PTPN22 mRNA was positively correlated with serum levels of ESR and CRP （r = 0.63, P = 0.005 ; r = 0.58, P 〈 0.01 ）. Also, the expression of PTPN22 mRNA was positively correlated with disease severity and extent of lesion （r = 0.51, P 〈 0.01; r = 0.44, P 〈 0.01 ）. The expression of PTPN22 mRNA in moderate to severe UC patients was significantly higher than that in mild UC patients （P = 0. 005）. The expression of PTPN22 mRNA in patients with extensive colitis was significantly higher than that in patients with left side colitis and proctitis （ P = 0. 029 ; P = 0.008）. Conclusions ： The expression of PTPN22 mRNA is correlated with UC activity, severity and extent of lesion. PTPN22 might play an important role in the pathogenesis of UC.

关 键 词：蛋白质酪氨酸磷酸酶 非受体型22 结肠炎 溃疡性 血沉 C反应蛋白质 T淋巴细胞 

分 类 号：R574.62[医药卫生—消化系统]