p38蛋白激酶(p38 MAPK)在癫大鼠脑内的表达  

The expression of p38 mitogen-activated protein kinase(p38 MAPK) in cerebra of rat with epilepsy

作　　者：王翠翠[1,2] 陈英辉[1,2] 

机构地区：[1]复旦大学附属金山医院神经内科,上海201508 [2]复旦大学上海医学院神经病学系,上海200032

出　　处：《复旦学报（医学版）》2013年第4期419-422,共4页Fudan University Journal of Medical Sciences

基　　金：上海市卫生局局级科研项目(2008080);上海市自然科学基金项目(09ZR1405500)~~

摘　　要：目的观察p38蛋白激酶(p38mitogen-activated protein kinase,p38MAPK)在癫大鼠脑内的表达情况。方法健康雄性SD大鼠随机分成正常对照组(n=8)和癫组(n=8)。采用戊四氮腹腔注射建立癫模型,大鼠点燃后的惊厥行为按照Racine的标准进行观察评分,采用Western blot和免疫荧光法比较两组大鼠脑内p38MAPK的表达情况。结果癫组大鼠脑内p38MAPK在皮层和海马的表达均显著高于正常对照组(P<0.01)。结论 p38MAPK在癫大鼠脑内表达上调。Objective To investigate the expression of p38 mitogen-activated protein kinase （p38 MAPK） in cerebra of rat with epilepsy. Methods Healthy male SD rats were randomly divided into normal （n = 8） and epilepuc group （n=8）. Epilepsy model was established with the intraperitoneal injection of pentylenetetrazol. Behaviors were classified according to the criteria of Racine. The expression of p38 MAPK in cerebra was observed by immunofluorescence and Western blot. Results Compared with the normal rats, the expression of p38 MAPK in cortex and hippocampus of epileptic rats was significantly higher （P〈 0.01）. Conclusions The expression of p38 MAPK was up-regulated in cerebra of epileptic rats.

关 键 词：p38蛋白激酶(p38 MAPK) 癫 戊四氮 P-糖蛋白 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]