阿托伐他汀在稳定兔动脉粥样硬化斑块和降低血栓事件中作用  

Role of atorvastatin in stabling artery plaque and reducing thrombotic events in rabbits

作　　者：庄须翠[1,2] 赵全明[1,2] 张明多[1,2] 赵欣[1,2] 冯婷婷[1,2] 赵学诚[1,2] 刘瑜[3] 于薇[4] 

机构地区：[1]首都医科大学附属北京安贞医院特需医疗科 [2]北京市心肺血管疾病研究所,北京100029 [3]首都医科大学基础医学院,北京100069 [4]首都医科大学附属北京安贞医院影像科,北京100029

出　　处：《中华实用诊断与治疗杂志》2013年第8期738-740,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(30972810;81071196);北京自然科学基金(7132078)

摘　　要：目的建立兔动脉粥样硬化易损斑块模型,评价阿托伐他汀对动脉粥样硬化斑块稳定性和血栓形成的影响。方法18只健康雄性大白兔以高脂饲养+球囊拉伤方法建立动脉粥样硬化模型后,随机分为高脂组和他汀组各9只,2组均给予间断高脂饲养,他汀组同时给予阿托伐他汀。药物激发实验后处死动物取其主动脉,行HE染色及免疫组织化学染色。结果高脂组各型动脉粥样硬化斑块比例分别为Ⅱ型5.29%、Ⅲ型13.94%、Ⅳ型26.44%、Ⅴ型25.00%、Ⅵ型29.33%;易损斑块主要有斑块面积大、纤维帽薄、帽核比值小并有较多炎性细胞浸润等病理学特点;他汀组易损斑块发生率(13.08%)较高脂组(29.33%)明显减少,稳定斑块发生率(86.92%)较高脂组(70.67%)增多,差异均有统计学意义(P<0.05);他汀组斑块面积占管腔总面积的百分比((23.48±9.94)%)和斑块厚度((311.27±190.98)μm)均较高脂组明显减小((50.23±10.08)%和(737.47±248.73)μm)(P<0.05);血栓发生率他汀组(33%)低于高脂组(75%)(P<0.05)。结论阿托伐他汀可改善兔动脉粥样硬化斑块稳定性,减少斑块破裂,降低血栓形成。Objective To establish rabbit models of atherosclerotic vulnerable plaques and evaluate the effect of atorvastatin on plaques stability and thrombosis. Methods Atherosclerotic vulnerable plaques were induced with high-fat feed and balloon injury in 18 male rabbits. All rabbits were randomly divided into high-fat group （n= 9） and atorvastatin group （n=9）, and were given high-fat diet, meanwhile atorvastatin was given to atorvastatin group. After pharmacological triggering, all survival rabbits were sacrificed to obtain the aorta and processed HE staining and immunohistochemical staining. Results The percentages of each model of atherosclerosis plaque were 5.29 % of type II , 13.94% of type III, 26.44% of type IV, 25.00% of type V , and 29.33± of type VI in high-fat group. Vulnerable plaques had large plaque area, thin fibrous cap, small lipid core ratio, and more infiltrating inflammatory cells. Compared with high-fat group, atorvastatin group had fewer vulnerable plaques （29. 33% vs 13.08%）, and more stable plaques （70.67% vs 86.92%） （P〈0.05）. The percentage of plaque area in total lumen area and plaque thickness were much smaller in atorvastatin group （（23. 48±9. 94）%, （311. 27±190. 98） μm） than those in high fat group （（50. 23 ± 10.08） %, （737.47±248.73）μm） （P〈0.05）. The thrombosis incidence was lower in atorvastatin group （33%） than that in high-fat group （75%） （P〈0.05）. Conclusion Atorvastatin can improve plaque stability and reduce plaque rupture and thrombosis.

关 键 词：动脉粥样硬化 易损斑块 兔 阿托伐他汀 

分 类 号：R543.5[医药卫生—心血管疾病]