水飞蓟宾葡甲胺对非酒精性脂肪肝大鼠的作用  

Protective effect of silybin meglumine on rats with non-alcoholic fatty liver disease

作　　者：冀润利[1] 张争运[1] 王毓麟[1] 

机构地区：[1]武警后勤学院附属医院肝胆胰脾科,天津300162

出　　处：《中华实用诊断与治疗杂志》2013年第8期771-772,776,共3页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的探讨水飞蓟宾葡甲胺(silybin meglumine,SM)对非酒精性脂肪肝大鼠的保护作用。方法 50只健康雄性SD大鼠随机分为5组,分别为空白对照组、脂肪肝模型组、SM低剂量组(100mg/kg)、SM高剂量组(300mg/kg)与阳性药对照组(熊去氧胆酸,60mg/kg),每组10只。高脂饮食饲养4周后开始给药,6周时检测血清谷丙转氨酶、谷草转氨酶、三酰甘油、总胆固醇和肝组织中肿瘤坏死因子-α和白细胞介素-8水平。结果 SM低、高剂量组和阳性药对照组大鼠血清谷丙转氨酶、谷草转氨酶、三酰甘油、总胆固醇水平较模型组明显降低(P<0.05),大鼠肝组织肿瘤坏死因子-α水平较模型组明显降低(P<0.05);SM高剂量组和阳性药对照组大鼠肝组织白细胞介素-8水平较模型组明显降低(P<0.05);SM低剂量组大鼠肝组织白细胞介素-8水平与模型组比较差异无统计学意义(P>0.05)。结论 SM对非酒精性脂肪肝大鼠有保护作用,可能与降低肿瘤坏死因子-α和白细胞介素-8的产生、减少炎性细胞浸润有关。Objective To investigate the protective effect of silybin meglumine （SM） on rats with non-alcoholic fatty liver disease. Methods Fifty male SD rats were randomly divided into 5 groups： normal group, model group, low-dose SM （100 mg/kg） group, high-dose SM （300 mg/kg） group and positive group （60 mg/kg ursodeoxycholic acid）, with 10 rats in each group. Drugs were administered after high-fat diet for four weeks, glutamic-pyruvic transaminase （GPT）, glutamic oxaloacetic transaminase （GOT）, triacylglycerol （TG） and total cholesterol （TC） in serum and tumor necrosis factor-α（TNF-α）, interleukin-8 （IL-8） in hepatic tissue were measured in 6 weeks. Results The levels of GPT, GOT, TG, TC and TNF-α were lower in low- and high-dose SM groups and positive group than those in model group （P〈 0.05）. IL-8 level was lower in high-dose SM group and positive group than that in model group （P〈0.05）. There was no significant difference in IL-8 level between low-dose SM group and model group （P〉0.05）. Conclusion SM has protective effect on rats with non-alcoholic fatty liver disease, which is correlated with decreasing the TNF-α and IL-8 levels and reducing inflammatory cell infiltration.

关 键 词：非酒精性脂肪肝 水飞蓟宾葡甲胺 熊去氧胆酸 

分 类 号：R575.5[医药卫生—消化系统]