精制抗放射性肺炎方的等效性研究  

Comparison of Therapeutic Effects between the Refined Extract and Crude Aquatic Solution of Anti-radiation Pneumonia Decoction

作　　者：欧瑶[1] 李曙光[1] 陈不尤[1] 周士忠[1] 肖明兵[1] 江枫[1] 钱霞[1] 陆亚鹏[2] 

机构地区：[1]南通大学附属医院,江苏南通226001 [2]南通大学海洋医学研究所,江苏南通226001

出　　处：《中国实验方剂学杂志》2013年第15期227-231,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省南通市社会发展计划(S2008035)

摘　　要：目的:观察抗放射性肺炎方(anti-radiation pneumonia decoction,ARPD)水提醇沉精制液和水煎剂治疗放射性肺损伤的等效性。方法:采用水提醇沉法初步精制ARPD水煎剂。将60只大鼠随机分为模型组、水煎剂组、精制液组和正常对照组。前3组均行直线加速器6MVX射线17Gy全胸单次照射。照射后每日分别给予ig生理盐水、ARPD水煎剂和精制液各35 g.kg-1,1次/d,每次10 mL.kg-1。各组大鼠分别于照射后第7,15,30,45,60天观察和取材,检测肺组织和血清中超氧化物歧化酶(SOD)、丙二醛(MDA)和羟脯氨酸(HYP)含量。结果:①模型组大鼠肺组织病理检查发现炎症改变及胶原增生,肺组织和血清SOD活力下降,而MDA和HYP升高,与同期对照组比有显著差异(P<0.01);②ARPD水煎剂组、精制液组两组肺组织病理检查炎症改变及胶原增生明显轻于同期模型组,两中药间无明显差异;③SOD活力下降,MDA和HYP升高,模型组甚于水煎剂组和精制液组(P<0.01),两中药制剂间无显著差异。结论:①早期应用ARPD可以明显减轻肺的放射性炎症反应,降低肺纤维化的程度;②ARPD精制液和水煎剂的抗放射性肺损伤疗效相当;③水提醇沉法是ARPD纯化精制的可行的工艺方法。Objective： Equivalence studys of refined extract and crude solution of anti-radiation pneumonia decoction（ARPD） on radiation-induced lung injury.Method： Refined extract of ARPD was made by alcohol purification from crude solution.Experiment group： model group,crude solution treatment group,refined extract treatment group and control group.Rats in model group,crude solution treatment group and refined extract treatment group were thoracically exposed to 6 MVX-rays at a dose of 17 Gy.The rats were treated with different solutions by gastrogavage once a day just after X-ray exposure.Rats in crude solution treatment group and refined extract treatment group were treated with 3.5 g.mL-1crude and refined ARPD respectively,and rats in model group and control group were gavaged with 0.9% sodium chloride,each time 10 mL.kg-1.The rats were sacrificed at the day of 7,15,30,45 and 60 after X-ray exposure.The levels of superoxide dismutase（SOD）,malondialdehyde（MDA） and hydroxyproline（HYP） in lung tissues and sera were measured simultaneously.Result： ①Pathological examination of lung tissue of model group revealed inflammatory changes and proliferation of collagenous fibers,in lung tissue and serum SOD activity decreased,while the content of HYP and MDA increased,much higher than the concurrent control group（P 0.01）,with a statistically significant difference;②The inflammatory reactions and collagenous proliferation in crude solution treatment group and refined extract treatment group were much slighter,compared with the same period of model group,but there was no difference between these two treatment groups;③The reduction of SOD activity and the rises of content of MDA and HYP in the model group severely greater than the crude solution treatment group and refined extract treatment group（P 0.01）,while no significant difference between two treatment groups.Conclusion： ① ARPD may reduce the inflammatory reaction of radiation-induced lung injury and reduce pulmonary fibrosis caused by ra

关 键 词：放射性肺损伤 抗放射性肺炎方 精制液 等效性 

分 类 号：R285.5[医药卫生—中药学]