槲皮素-7-硫酸酯钠对H_2O_2所致DNA氧化损伤的保护作用  

Protective Effect of Sodium Quercetin-7-mono-sulfonate on H_2O_2 Induced DNA Oxidative Damage

作　　者：陈再蓉[1] 赵小燕[2] 朱玉新[1] 沈渠深[2] 戴忠[2] 

机构地区：[1]新疆阿克苏职业技术学院医学系,新疆阿克苏843000 [2]广东医学院药理学教研室,广东东莞523808

出　　处：《中国实验方剂学杂志》2013年第15期243-246,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨槲皮素-7-硫酸酯钠的体外抗氧化活性及对DNA氧化损伤的保护作用。方法:通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基(.DPPH)、羟自由基(OH.)和超氧阴离子自由基(O2-·)清除实验评价槲皮素-7-硫酸酯钠的体外抗氧化活性;采用紫外扫描法、凝胶电泳技术观察槲皮素-7-硫酸酯钠对H2O2所致DNA氧化损伤的保护作用。结果:自由基清除实验结果显示,槲皮素-7-硫酸酯钠能有效清除.DPPH、·OH和O2-·自由基,其清除能力较维生素C(VC)及槲皮素都强,且呈浓度依赖性。DNA氧化损伤保护实验结果显示,H2O2可致小牛胸腺DNA出现断裂梯带和浓度下降,槲皮素-7-硫酸酯钠预处理可抑制H2O2所致的小牛胸腺DNA断裂及浓度下降,且有浓度依赖性。结论:槲皮素-7-硫酸酯钠体外有较强的抗氧化能力,并对H2O2所致的DNA氧化损伤有保护作用。Objective： To provide a theoretical basis for clinical application of sodium quercetin-7-monosulfonate（SQMS）,its antioxidant property and DNA oxidative damage protective effect in vitro were studied.Method： The antioxidant activity of SQMS was evaluated by 1,1-diphenyl-2-picryl-hydrazyl（.DPPH）,hydroxyl radical（OH·） and superoxide radical（·O2-） scavenging assays.Gel electrophoresis and spectrophotometry methods were used to study the protective effect of SQMS on H2O2 induced DNA oxidative damage in vitro.Result： SQMS exhibited a concentration-dependent scavenging effect on.DPPH,.OH and O2-.,and its scavenging effects are stronger than vitamin C（VC） and quercetin.By gel electrophoresis and spectrophotometry,the DNA ladder disappeared and the DNA concentration obvious increased by treatment with H2O2.Pretreatment with quercetin or sodium quercetin-7-sulfonate,the DNA ladder was more clear and the concentration of DNA decreased obviously.Conclusion： SQMS has stronger antioxidant activity in vitro,and has protective effect on H2O2induced DNA oxidative damage.

关 键 词：槲皮素-7-硫酸酯钠 体外 抗氧化活性 DNA氧化损伤 

分 类 号：R285.5[医药卫生—中药学]