原代乳小鼠心肌细胞的分离培养及鉴定  

Isolation,culture and identification of neonatal mouse cardiomyocytes

作　　者：程阔菊[1] 黄河[1] 杜智勇[1] 李洪[1] 杨天德[1] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆400037

出　　处：《重庆医学》2013年第22期2638-2640,共3页Chongqing medicine

基　　金：国家自然科学基金面上项目(30871061)

摘　　要：目的建立原代乳小鼠心肌细胞的培养方法,评价心肌细胞的存活状况并鉴定心肌细胞纯度。方法应用胰酶联合胶原酶共同消化3d内C57乳小鼠心脏,台盼蓝染色判断心肌细胞存活率,α-actin免疫荧光染色鉴定心肌细胞纯度。结果利用3d内乳鼠心脏可成功培养原代小鼠心肌细胞,台盼蓝染色显示细胞存活率大于95%,α-actin免疫荧光染色显示心肌细胞纯度达95%以上。结论严格控制实验条件,可成功培养高存活率和高纯度的小鼠心肌细胞。Objective To establish a method to isolate,culture and identify cardiomyocytes from neonatal mouse,estimate the cardiomyocytes survival rate,and identify the purity of cardiomyocytes.Methods Neonatal C57 mouse heart（within 3days）was digested using trypsin and collagenase.Cardiomyocytes survival rate was estimated by trypan blue staining,and cardiomyocytes purity was identified byα-actin immunofluorescence staining.Results Mouse cardiomyocytes could be successfully isolated and cultured using neonatal mouse within 3 days.Trypan blue staining showed cardiomyocytes survival rate was95%,and cardiomyocytes purity was more than 95% demonstrated byα-actin immunofluorescence staining.Conclusion Under the strict experimental conditions,mouse cardiomyocytes can be successfully isolated and cultured with high survival rate and high purity.

关 键 词：乳小鼠 心肌细胞 分离 纯化 培养 鉴定 

分 类 号：R-332[医药卫生]