进境船舶压舱水中一株石莼属绿藻的种类鉴定  

Identification of Ulva prolifera (Chlorophyta,Ulvales) transported by entry ballast water

作　　者：陈先锋[1] 段维军[1] 高姗姗[2] 徐年军[2] 张慧丽[1] 陈炯[2] 朱水芳[3] 

机构地区：[1]宁波出入境检验检疫局检验检疫技术中心,浙江宁波315012 [2]宁波大学 [3]中国检验检疫科学研究院植物检疫研究所

出　　处：《植物检疫》2013年第4期48-53,共6页Plant Quarantine

基　　金：国家自然科学基金(41276122);国家质检总局科研项目(2011IK181);浙江省科技创新团队项目(2010R50029);中国检科院科研项目(2009JK002);宁波市社会发展科研项目(2011C50082)

摘　　要：本研究采用形态学观察结合核糖体ITS、叶绿体rbcL基因和5S rDNA间隔序列3个分子标记系统进化分析的研究方法,对来自宁波梅山港的进境船舶压舱水中的一株石莼属绿藻进行了种类鉴定。形态学观察结果表明其具有藻体中空管状、具分枝、中部细胞呈典型纵向排列细胞、无明显的圆角和帽状叶绿体、每个细胞中有蛋白核1个到2个,少数细胞具有3个蛋白核的形态特征。ITS和rbcL序列分析结果均将其划分在LPP分枝,5S rDNA间隔序列分析表明该绿藻属于U.prolifera分枝。综合以上结果,将其鉴定为浒苔U.prolifera,这是国内外首次报道浒苔可通过压舱水进行传播的报道,也是我国首次从进境压舱水中截获浒苔这一绿潮藻。In this study,the ballast waters coming from Busan of South Korea have been collected in the Meishan harbour of Ningbo port（China）.One Ulva sp.sample has been cultured in the collected ballast water.The taxonomic status of the Ulva sample from the ballast was assessed by using both morphological and molecular（ITS and rbcL sequences） data.In addition,5S rDNA analyses were performed for the LPP clade.At the morphological level,the algae with a flat central cavity,often richly branched,one to three pyrenoids per cell,and without distinctly rounded corners and cap-like chloroplasts in the cell.At the molecular level,the isolate has been identified as U.prolifera.According to these results,the sample was identified as U.prolifera O.F.Müller.To our knowledge,this is the first report of U.prolifera transported by imported ballast water;this is also the first report of intercepted U.prolifera from entry ballast water in China.

关 键 词：压舱水 浒苔 形态学 系统进化 鉴定 

分 类 号：S41-30[农业科学—植物保护]