环境16S rDNA和16S rcDNA序列分析湖光岩玛珥湖的浮游细菌组成  

Bacterioplankton Composition in Huguangyan Maar Lake Revealed by Environmental 16S rDNA and 16S rcDNA Sequence Analysis

作　　者：秦青英[1] 曾永辉[1] 郭倩茹[1] 简纪常[1] 鲁义善[1] 吴灶和[1,2] 

机构地区：[1]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088 [2]仲恺农业工程学院,广东广州510000

出　　处：《广东海洋大学学报》2013年第3期1-9,共9页Journal of Guangdong Ocean University

基　　金：国家自然科学基金青年项目(No.30900045);广东省自然科学基金博士启动项目(No.B09292)

摘　　要：通过提取环境总16S rDNA和总16S rRNA分别构建DNA克隆文库和反转录cDNA文库,并进行克隆测序和序列分析,探讨湖光岩玛珥湖浮游细菌和浮游活性细菌的遗传多样性。结果表明：构建两个水层的4个克隆文库,即1 m和10 m水层总菌的16S rDNA文库和总活性菌的16S rcDNA文库,共获得413条序列,分属15门、32目、52科;1 m水层的rDNA文库与rcDNA文库中Verrucomicrobia类群所占比例最大（36.8%和32.1%）,其次为Alphaproteobacteri（a 15.1%和16%）和Betaproteobacteri（a 17.9%和16%）;在10 m水层的2个文库中,Alphaproteobacteria类群均占绝对优势,分别为63.5%（rDNA）和43.8%（rcDNA）,其次为Verrucomicrobia（11.5%和14.3%）,其他细菌类群包括Acidobacteria、Chloroflexi、Firmicutes、待定门OD1、Planctomycetes、Spirochetes和Deltaproteobacteria,比例在0.8%~3.8%之间;在科的水平上,SAR11为湖光岩玛珥湖最为丰富的类群,占总克隆数的26.9%,其中在10 m水层的比例高达51.5%。序列同源性分析表明,绝大多数序列（89.8%）来源于新种,其中19.6%的序列可能来自于新属,69%的序列可能来自于全新的科。湖光岩玛珥湖浮游细菌群落结构较为独特,有大量的浮游细菌种类尚未获得相应的纯培养物,蕴含着丰富而新颖的微生物资源。Huguangyan Maar Lake belongs to a unique type of volcanic lake.Little is known about the microbial diversity in Maar Lake.So it is important to study the bacterial diversity in the water body for protecting and developing Maar Lake microbial resources.Total DNA and RNA were extracted from planktonic microbial communities in the Huguangyan Maar Lake,and 16S rDNA and 16S rcDNA clone libraries were constructed,and a number of clones were sequenced with the aim to analyze the genetic diversity of total bacterioplankton and active planktonic bacteria.Four clone libraries were constructed from the community DNA and cDNA of the 1 m and 10 m layers of the Huguangyan Maar Lake with 413 sequences reported,which were further classified into 15 phyla,32 orders,and 52 families.Within the 16S rDNA library and 16S rcDNA library of the 1m layer,Verrucomicrobia occupied the largest proportion（36.8% and 32.1%,respectively）,followed by Alphaproteobacteria（15.1% and 16%,respectively） and Betaproteobacteria（17.9% and 16%,respectively）.Alphaproteobacteria represented the most abundant taxa within the two libraries of the 10 m layer with the proportions of 63.5%（rDNA） and 43.8%（rcDNA）,respectively,followed by Verrucomicrobia（11.5% and 14.3%）.Other bacterial groups include Acidobacteria,Chloroflexi,Firmicutes,candidate phylum OD1,Planctomycetes,Spirochetes and Deltaproteobacteria,with a proportion of 0.8~3.8% documented.At the family level,SAR11 was the most abundant group in the bacterioplankton of the Huguangyan Maar Lake,accounting for 26.9% in term of the number of clones and reaching a maximum of 51.5% at the 10 m layer.Sequence similarity analysis shows that most of the sequence（89.8%） may originate from novel species,of which 19.6% may come from novel genera and 69% may be derived from novel families.The community structure of bacterioplankton in the Huguangyan Maar Lake is very unique in terms of both diversity and abundance of bacterial groups.A large number of 16S rDNA and rcDNA clones do not have c

关 键 词：湖光岩玛珥湖 浮游细菌 浮游活性菌 16S rDNA 16S RRNA 系统发育分析 遗传多样性 

分 类 号：Q939.19[生物学—微生物学]