14-3-3zeta与p-Bad在T1期非小细胞肺癌中的表达及意义  

Expression of 14-3-3zeta and p-Bad proteins in stage T_1 non-small cell lung cancer and the significance

作　　者：臧东钰[1] 李福智[1] 杨春雨 

机构地区：[1]辽宁医学院附属第三医院胸外科 [2]附属第一医院病理科,辽宁锦州121001

出　　处：《解剖学报》2013年第4期509-513,共5页Acta Anatomica Sinica

基　　金：辽宁省自然科学基金项目(201202135)

摘　　要：目的观察14-3-3zeta和p-Bad在T1期非小细胞肺癌(NSCLC)中的表达,探讨两者在非小细胞肺癌发展中的作用及相互关系。方法取110例非小细胞肺癌及癌旁正常肺组织存档蜡块和50例新鲜NSCLC及癌旁正常肺组织标本,采用免疫组织化学和免疫印迹法(Western blotting)检测14-3-3zeta和p-Bad蛋白的表达。结果与正常肺组织相比,14-3-3zeta在NSCLC中的表达显著增强,与肺癌的分化程度和淋巴结转移有关,而与患者的年龄、性别及病理学分型无关。p-Bad在正常肺组织中轻微表达,而在NSCLC中表达显著增强。且其表达与患者的年龄、性别及病理学分型无关,但与癌组织分化程度、淋巴结转移相关。结论 14-3-3zeta蛋白与p-Bad与T1期NSCLC发展、侵袭和转移密切相关。Objective To observe the expression of 14-3-3zeta and p-Bad in stage T1 non-small cell lung cancer （NSCLC） , and to investigate the relationship between the expression of 14-3-3zeta and p-Bad in NSCLC. Methods One hundred and ten paraffin blocks and 50 fresh samples from NSCLC patients and normal lung were observed. Expression of 14-3-3zeta and p-Bad were detected by immunohistochemistry and Western blotting. Results Up-regulation of 14-3-3zeta was observed significantly in stage Tl NSCLC in contrast to that in the normal lung. The increased 14-3-3zeta protein level was correlated with differentiation degree and lymph node metastasis, but it was disassociated with age, gender and histological type. Decreased expression of p-Bad was observed in NSCLC in contrast to that in the normal lung. Expression of p-Bad was related to differentiation degree and lymph node metastasis, but it was disassociated with age, gender and histological type. Conclusion 14-3-3zeta and p-Bad play an important role in development and progression of NSCLC.

关 键 词：非小细胞肺癌 14-3—3zeta p-Bad 免疫印迹法 人 

分 类 号：R734.2[医药卫生—肿瘤]