右旋糖酐-二乙烯三胺五乙酸-钆离子螯合物与马根维显在间质磁共振淋巴造影中的对比  

Comparative study of the dextran-DTPA-Gd and gadopentetate dimegiumine in interstitial magnetic resonance lymphography

作　　者：吴晶[1] 孙玥[2] 万升标[2] 刘世恩[3] 冯元勇[1] 李薇[4] 徐文坚[3] 江涛[2] 尚伟[1] 

机构地区：[1]青岛大学医学院附属医院口腔颌面外科,青岛266003 [2]中国海洋大学医药学院,青岛266003 [3]青岛大学医学院附属医院放射科,青岛266003 [4]青岛大学医学院附属医院耳鼻喉头颈外科,青岛266003

出　　处：《解剖学报》2013年第4期570-575,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(21171154);教育部留学回国人员科研启动基金资助项目[教外司留(2004)527号]

摘　　要：目的比较右旋糖酐-二乙烯三胺五乙酸-钆离子螯合物(dextran-DTPA-Gd)和马根维显两种对比剂在间质MRI淋巴造影中淋巴显影的价值。方法选择健康成年新西兰兔12只,体重为2.7～3.7kg。仰卧位固定兔,经3D TOF CE-MRA序列扫描,平扫后右侧后肢第1、2、3趾蹼间隙注射dextran-DTPA-Gd各0.4ml(3.96×10-3mol/L),共1.2ml;30min后左侧后肢第1、2、3趾蹼间隙注射马根维显各0.4ml(0.4998mol/L),共1.2ml,行MR增强扫描,扫描间隔分别为10、15、20、25、30、35、40、45、50、55、60min、2h、4h、24h。平扫和增强扫描的相关参数相同。分析不同时间段淋巴显影强化程度,测量并计算腘窝淋巴结增强前后的信号强度(E%),绘制其信号强度-时间曲线,比较两种显影剂对淋巴显影的区别。结果平扫时双侧腘窝淋巴结均呈等信号。右侧dextran-DTPA-Gd注射后10min,引流区后肢淋巴管及腘窝淋巴结信号强化明显、显示清晰,腘窝淋巴结E%为212.7%,35min左右达到峰值,E%值为314.1%,4h后为208.2%,24h后扩清。左侧马根维显注射10min时,引流区域后肢血管强化明显,造影剂大部分吸收入血管进入膀胱,后肢淋巴管及腘窝淋巴结信号弱,腘窝淋巴结E%为78.8%,20min左右达到峰值,E%值为98.3%,4h后减至29.0%,24h后扩清。淋巴结E%经统计学分析差异有统计学意义(P<0.05)。实验前后动物的生化检查等数据未见异常变化,其差异无统计学意义(P>0.05),送检的内脏器官组织学检查未见明显的病理学改变。结论相比马根维显小分子造影剂,dextran-DTPA-Gd是一种有效的淋巴造影剂,能够特异性靶向强化淋巴结及淋巴管。Objective To compare the feasibility of interstitial magnetic resonance （MR） lymphography using subcutaneous injection of dextran-DTPA-Gd and gadopentetate dimeglumine. Methods The 1.2ml dextran-DTPA-Gd （3.96×10 -3 mol/L） was injected subcutaneously into the interdigital skin fold of the dorsal aspect of the right hind leg in 12 New Zealand rabbits. The 1.2ml gadopentetate dimeglumine（0. 4998mol/L） was injected into the left hind leg in the same rabbit after 30 minutes. MR images were obtained before injection and at 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes and 2,4,24 hours after injection by the 3D TOF CE-MRA sequence. The signal intensities of popliteal lymph node were measured, the enhancing rates（ E% ） were calculated and the signal-time course curve was drawn. Results The bilateral popliteal lymph node showed the same signal intensities. The lymphatic vessels in the right hind limb drainage area and popliteal lymph node were strengthened significantly and the E% of popliteal lymph node was 212.7% at 10 min after injecting dextran-DTPA-Gd, reached the peak（314.1% ） by 35min, was 208.2% at 4 hours and disappeared 24 hours later. The signal intensities of the left lymphatic and popliteal lymph node were weak and the E% of popliteal lymph node was 78.8% 10 min after injecting gadopentetate dimeglumine, reached the peak （98.3%） by 20min, was 29.0% 4 hours, and disappeared 24 hours later. The E% was statistically significant（P 〈 0. 05）. Animals in each group during the observation period were no significant adverse reactions, biochemical examinations were not statistically significant （P 〉 0.05）. Histological examination of the injected organs showed no obvious pathological changes. Conclusion Compared with small molecular gadopentetate dimeglumine, the dextran-DTPA-Gd is an effective lymphatic contrast agent, which can specific strengthen the lymph nodes and lymphatic.

关 键 词：磁共振成像 淋巴造影术 钆 兔 

分 类 号：R739.81[医药卫生—肿瘤]