视黄酸和三碘甲腺原氨酸在减轻睡眠剥夺损害认知功能中的作用  

Effects of retinol acid and triiodothyronine on alleviating the impairment of cognitive function by sleep deprivation

作　　者：张娜[1] 马强[1] 陈学伟[1] 徐传香[1] 安改红[1] 崔博[1] 佘晓俊[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国应用生理学杂志》2013年第4期294-297,300,共5页Chinese Journal of Applied Physiology

摘　　要：目的:探讨外源性补充视黄酸(RA)或三碘甲腺原氨酸(T3)在减轻睡眠剥夺(SD)损害认知功能中的作用。方法:雄性Wistar大鼠随机分为4组(n=6):即对照组(C组),睡眠剥夺组(SD组),睡眠剥夺+RA组(SD+RA组)和睡眠剥夺+T3组(SD+T3组)。利用旷场实验观察大鼠的神经行为,在体脑立体定位电生理法测量大鼠海马齿状回长时程增强(LTP),Western blot法检测海马Ng蛋白表达量。结果:与SD组比较,SD+RA和SD+T3组大鼠在旷场中穿行格数显著增加(P<0.05),海马齿状回PS峰升幅显著增高(P<0.05,P<0.01),海马Ng蛋白表达水平也显著升高(P<0.05),且均与C组无显著性差异。结论:RA和T3可以通过上调海马中Ng蛋白的水平,从而有效缓解SD所致的神经系统的抑制状态。Objective： To explore the effects of retinol acid（RA） and triiodothyronine（T3） on alleviating the impairment of cognitive function by sleep deprivation（SD）.Methods： Male Wistar rats were divided into 4 groups： control group（C group）,sleep deprivation group（SD group）,sleep deprivation ＋ RA group（SD＋RA group） and sleep deprivation ＋ T3 group（SD＋T3 group）.Open field test（OFT） was used to observe the nervous behavior of the rats after SD and electrophysiological brain stereotactic method was used to test long-term potentiation（LTP） in dentate gyrus（DG） of the rats.Ng protein expression was determined by Western blot.Results： Compared with the SD group,the number of crossing in OFT,the changes of amplitude of population spike（PS） and the expression of Ng protein in hippocampus were higher significantly in the SD＋RA and SD＋T3 groups.All of these had not significant difference comparing with the C group.Conclusion： RA and T3 may alleviate the restrain state of neural system after SD by augmenting the expression of Ng protein in hippocampus.

关 键 词：视黄酸 三碘甲腺原氨酸 睡眠剥夺 认知功能 神经颗粒素 

分 类 号：R733[医药卫生—肿瘤]