肝癌患者发病前血清中高尔基体糖蛋白73水平的回顾性动态观察  

Retrospective observation of dynamic levels of serum Golgi protein 73 in patients prior to the onset of liver cancer

作　　者：陈建国[1] 陆卫中[1] 朱源荣[1] 张永辉[1] 陆建华[1] 陈陶阳[1] 

机构地区：[1]江苏省启东肝癌防治研究所病因研究室,226200

出　　处：《中华预防医学杂志》2013年第8期731-735,共5页Chinese Journal of Preventive Medicine

基　　金：卫生部疾病预防控制局、中国癌症基金会项目（2005-004）;国家科技重大专项（2012ZX10002009018,2012ZX10002010）

摘　　要：目的探讨肝癌发病前后高尔基体糖蛋白73（GP73）的动态水平及相关因素。方法2007至2012年对一组乙型肝炎表面抗原（HBsAg）阳性者人群开展一年两次的周期性的筛查，统一保留血标本于生物样本库，直至肝癌发生。将肝癌发病时及发病前30个月内6个时点中，至少有3次血标本并有B超临床检查结果的39例肝癌患者列为血清GP73检测分析对象，最终获得162个标本。采用双抗体夹心ELISA法检测GP73。用stata软件作时序间分析并进行不同分组间差异的统计学检验。结果肝癌发病时的39例患者GP73检测值为（126．77±73．73）μg／L，发病前5次GP73检测的平均值分别为（128．32±81．18）、（129．97±83．62）、（127．38±80．10）、（135．52±97．88）及（138．24±93．58）μg／L，差异无统计学意义（F=0．07，P=0．997）。39例肝癌发病前后的GP73水平未见明显的趋势变化。162个检测样本按B超检查结果划分为不伴有肝硬化（63例次）和伴有肝硬化（99例次）两组，GP73平均水平分别为（97．16±51．39）、（151．20±91．68）μg/L，差异有统计学意义（F=18．22，P〈0．01）。进一步以GP73均值（130．19μg／L）将调查对象分为两组，不伴有肝硬化者中只有1／14的患者GP73水平高于平均值，而伴有肝硬化者（25例）中有12例的GP73水平高于平均值，差异有统计学意义（P=0．013）。拟合回归模型也显示GP73与时间序列不相关（t=0．75，P=0．455），而与肝硬化相关（t=4．30，P〈0．01）。结论肝癌患者发病时及发病前30个月内的GP73水平动态变化不大。肝癌患者GP73水平的高低是由肝病背景所决定的；肝硬化可能是一个主要的影响因素或混杂因素。Objective To observe the dynamic levels of serum Golgi protein 73 （GP73） in patients prior to and after the onset of liver cancer, and to explore the related factors. Methods From 2007 to 2012, a periodical screening program was carried out in a group of high risk population with positive Hepatitis B surface antigens （HBsAg）, twice a year. Their serum specimens from every screening time point were kept in Qidong Biobank until liver cancer was diagnosed. Thirty-nine patients with liver cancer were recruited for the study, each of them at least had three times of specimens collected as well as B untrasound scan （BUS） exam results at onset of disease and within 30 months before diagnosed, amongst 6 time points. In total, there were 162 specimens collected to test GP73 by double-antibody sandwich enzyme-linked immuno-sorbent assay （ELISA）. Statistical analyses of time series and differences among groups were performed by stata software 10. Results The average value of 39 patient＇s GP73 at the time point of liver cancer onset was （ 126. 77±73.73） μg/L, while the values at the other five time points prior to the onset were （ 128.32± 81.18）,（129.97 ±83.62）, （127.38 ±80.10）, （135.52 ±97.88） and （138.24 ±93.58） μg/L, respectively, with no significant difference （ F = O. 07, P = O. 997 ）. No obvious changing trends of GP73 were observed among the 39 liver cancer cases at the 6 time points. All 162 samples were divided into two groups： without hepatie cirrhosis （63 samples） and with cirrhosis （99 smnples） aceording to findings of B-uhrasonie wave; whose average GI3 values were separately （97. 16 ± 5l. 39） and （151.20 ± 91.68） μg/L. The diftrenee showed statistical significanee （ F = 18.22, P 〈 0. 01 ）. Furthermore, if we grouped the samples by the average value of GP73 at 130. 19 μg/L, then there were only 1/14 of the subjects without hepatic cirrhosis having higher GP73 values, but 12 of the 25 subjects with hepatic cirrhosis having higher GP

关 键 词：肝肿瘤 肝硬化 高尔基体 肝炎表面抗原 乙型 时间序列 

分 类 号：R735.7[医药卫生—肿瘤]