四物汤组方配伍主要成分的HPLC/DAD定量分析  

Simultaneous determination of major compounds in Siwu Decoction and its component parts by HPLC/DAD

作　　者：朱敏 段金廒 刘培 宿树兰 唐于平 

机构地区：[1]南京中医药大学附属医院,南京210036 [2]江苏省方剂高技术研究重点实验室,南京210046

出　　处：《中华中医药杂志》2013年第8期2385-2389,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技支撑计划(No.2008BAI51B01);国家自然科学基金(No.81274058);国家教育部"新世纪优秀人才支持计划"(No.NCET-09-0163);江苏省自然科学基金(No.BK2012489);江苏省高校自然科学重大基础研究项目(No.06KJA36022;No.10KJA360039);江苏高等学校优秀科技创新团队支持计划(2009年度);江苏高校优势学科建设工程资助项目(No.ysxk-2010)~~

摘　　要：目的:从四物汤及其组方药味、药对、药组中主要成分的定量比较分析四物汤组方配伍的物质组成特点,为四物汤组方配伍规律的研究提供依据。方法:采用HPLC/DAD法测定,色谱柱:Waters SunFire-C18柱(4.6mm×250mm,5μm);柱温:(30±1)℃;流动相:黄乙腈(A)-0.1%磷酸水(B);流速:0.8mL/min;梯度洗脱。结果:药对当归-川芎,当归-白芍,当归-熟地黄和白芍-川芎中阿魏酸和洋川芎内酯I的含量均高于其组成单味药;药对当归-白芍,白芍-川芎,白芍-熟地黄中芍药苷含量均高于单味药白芍;四物汤全方中阿魏酸,洋川芎内酯I和芍药苷的含量均高于组方单味药;没食子酸和绿原酸在组方前后的含量则没有特定规律;药味,药对,药组和全方中5-羟甲基糠醛(5-HMF)含量均低于单味药熟地黄。结论:四物汤及其药对的配伍具有促进或抑制所含成分溶出的作用,为进一步揭示四物汤功效物质基础及组方配伍特点提供科学依据。Quantitation some major components in Siwu Decoction and its composite medicine, medicine- pairs and medicine-groups to provide evidence for the illustration of Siwu Decoction＇s compatible regularity. Methods： HPLC/ PDA was used. The column was Waters sunfire-C18 （4.6mm×250mm, 5μm） and the column temperature was set at （30±1）℃; The mobile phase was methyl cyanides （A）-0.1% phosphoric acid aqueous solution （B） and the flow rate was set at 0.8mL/min with gradient elution. Results： The contents of ferulic acid and senkyunolide I in medicine-pairs of Angelicae Sinensis Radix （ASR）- Chuanxiong Rhizoma （CR）, ASR-Paeoniae Radix Alba （PRA）, ASR-Rehmanniae Radix Praeparata （RRP）, PRA-CR all increased compared with the single medicine of ASR, CR, PRA and RRP; The contents of ferulic acid, senkyunolide I and peoniflorin in Siwu decoction were higher than that of single medicine; The contents of gallic acid and chlorogenic acid in single medicine, medicine-pairs, medicine-groups and Siwu Decoction exhibited undefined regularity; The single medicine of RRP exhibited the most content of 5-HMF than medicine-pairs, medicine-groups and the whole formula. Conclusion： The compatibility of the formula and medicine-pairs had the effect of promoting or inhibiting the dissolution of some major components. The present research provided a reliable evidence for the illustration of chemical basis and compatibility regularity of Siwu Decoction.

关 键 词：四物汤 药对 配伍 有机酸 芍药苷 5-羟甲基糠醛 川芎内酯 

分 类 号：R284[医药卫生—中药学]